摘要
【目的】探讨反义抑制C-myc蛋白的表达对人胆管癌细胞QBC939体外增殖的影响。【方法】采用常规方法培养QBC939细胞，合成C-mycASODN及Ns0DN，并将其转染QBC939细胞；用Western-blot法检测转染AS（）DN组（转染组）、转染NSODN组（无义组）及空白对照组细胞中c-myc蛋白的表达；通过四甲基噻唑蓝（MTT）实验检测各组细胞在体外的存活率。【结果】无义组与空白对照组灰度值比较无显著性差异（P〉0．05）；转染组的灰度值显著低于空白对照组（P〈0．01）；转染不同浓度（Mmol／L）的cmycASODN后，各组细胞的存活率分别为：0．5组为79．6％、1．0组为75．0％、2．0组为70．9％、4．0组为59．1％、8．0组为47．3％，均显著低于空白对照组（P〈0．01）。【结论】反义抑制c-myc蛋白的表达后，人胆管癌细胞在体外的增殖受到了抑制，且其抑制作用随着c-mycASODN浓度的增加而增强。
[Objective] To explore the effect of antisense inhibition of C-myc protein expression on the pro liferation of human bile duct cancer（QBC939） cells in vitro. [Methods] QBC939 cell was cultured by using the routine method. C-myc ASODN and NSODN were designed and transfeeted into QBC939 cells. Western-blot was used to detect the expression of c-myc in ASODN group（transfection group）, NSODN group（nonsense group） and control group. The survival rate of QBC939 cell in vitro was detected by MTT assay. [Results] There was no significant difference in the grayscale value between NSODN group and control group （ P 〉 0.05）, and the grayscale value in ASODN group was significantly lower than that of control group（ P〈0.01）. The cell survival rate after the transfection with different concentration of C-myc ASODN in each group was 79.6＇%（0.5μmol/L group）, 75. 0%0（1.0 μmol/L group）, 70. 9%（2.0 μmol/L group）, 59. 1%（4.0 /zmol/L group） and 47.3%（8.0/amol/L group）, respectively, which were all significantly lower than those in the control group（ P〈0.01）. [Conclusion] The antisense inhibition of C myc protein expression can inhibit the proliferation of human bile duct cells in vitro. The inhibition increases with the concentration of C-myc ASODN.
出处
《医学临床研究》
CAS
2011年第6期1070-1072,共3页
Journal of Clinical Research
