摘要
采用正交试验设计对菌株WS4006-7E-NUT-21 L-苏氨酸发酵的种子培养基进行优化,同时对影响种子生长的条件如种子培养时间、种子液初始pH值、种子装液量等进行了研究,最终确定了L-苏氨酸发酵的最优种子培养条件。确定了种子培养基的最佳组成为葡萄糖30 g/L、硫酸铵3 g/L、玉米浆40 g/L、酵母膏5 g/L;种子的最佳培养条件为250 mL的三角瓶中装液量25 mL,种子培养基的初始pH值7.0,培养时间16 h,接种量10%。
The seed culture medium of L-threonine producing strain WS4006-7E-NUT-21 was optimized by orthogonal design.Meanwhile,the factors that might influence the growth of seeds such as cultivation time,pH,and culture volume were studied;and the optimum cultivation condition was obtained.The optimal medium composition was 30 g/L glucose,3 g/L ammonium sulfate,40 g/L corn steep and 5 g/L yeast extract.While the best seed cultivation conditions were cultivating 25 mL liquid seed in 250 mL of flask,originate pH 7.0,cultivation time 16 h and with an inoculation amount of 10%.
出处
《湖北农业科学》
北大核心
2011年第13期2730-2733,共4页
Hubei Agricultural Sciences
基金
江苏畜牧兽医职业技术学院院长基金项目
关键词
L-苏氨酸
发酵
种子培养基
培养条件
优化
L-threonine
fermentation
seed culture medium
culture conditions
optimization
