摘要
目的:研究姜黄素对肺纤维化大鼠肺成纤维细胞增殖、凋亡的影响,探讨凋亡诱导因子(AIF)在肺成纤维细胞凋亡中的作用。方法:将体外培养的肺纤维化大鼠成纤维细胞,分别于不同浓度的姜黄素(5、10、20、40μM)和caspase-3抑制剂Z-DEVD-fmk(20μM)孵育,观测细胞生长状态变化。MTT检测成纤维细胞增殖抑制率;流式细胞仪检测细胞凋亡率;Western-Blot测定凋亡诱导因子(AIF)蛋白表达及核转位结果:流式细胞术检测细胞凋亡,5～40μM姜黄素处理12 h,其凋亡率呈浓度依赖,对照组相比,差异显著;而抑制caspase-3并不能完全阻止细胞凋亡。Western-Blot结果显示,姜黄素处理组出现凋亡诱导因子(AIF)蛋白表达与核转位,抑制caspase-3活性后未检测出AIF表达结论:姜黄素可抑制肺成纤维细胞增殖,其诱导大鼠肺成纤维细胞凋亡,可能与线粒体释放AIF有关。
Objective： To the effect of curcumin on proliferation and apoptosis and the mechanism of apoptosis-inducing factor（AIF） on rat pulmonary fibroblasts.Methords： Fibroblasts derived from lung tissue of rat were cultured in vitro.Cultured cells were ex-posed to curcumin with different final concentration（5 μM,10 μM,20 μM,40 μM） and Z-DEVD-fmk with final concentration（20 μM）.MTT assay was used to determine the inhibitory rate of curcumin on the proliferation of cells.Flow cytometry identified the apop-tosis.Western-blot was used to determine the expression and nuclear translocation of AIF protein.Results： Curcumin inhibited prolifera-tion of pulmonary fibroblasts in a dose-dependent manner（5 μM,10 μM,20 μM,40 μM）,which was detected by Flow cytometry.But the pro-liferation inhibitory and the apoptosis induced by curcumin were not inhibited by Z-DEVD-fmk.In curcumin treated groups,AIF was ob-served associated with the nuclear translocation.Conclusion： Curcumin inhibited proliferation of pulmonary fibroblasts.The cur-cumin-induced apoptosis may be associated with the releasing of AIF from mitochondria.
出处
《现代生物医学进展》
CAS
2011年第14期2654-2657,共4页
Progress in Modern Biomedicine
