摘要
目的建立乳鼠感染的模型,检测轮状病毒（Rotavirus,RV）肠道内、外感染乳鼠肝脏IFN-γ和IL-10水平,比较肝脏IFN-γ和IL-10在轮状病毒肠道内外感染,以及不同时间点变化的差异性,进一步探讨IFN-γ和IL-10免疫自稳失衡与轮状病毒肠道外感染发病的关系,推测以恢复细胞因子平衡为目标的治疗策略可能是治疗轮状病毒肠炎的一种新方法。方法实验动物选用35日龄的清洁级BALB/C乳鼠,将54只乳鼠随机分为3组,每组18只,即实验组,包括肠道外组：通过腹腔注入0.10 mL（1×10-5）TCID50感染性滴度计量的SA-11株病毒;肠道内组：通过口腔灌入0.10 mL相同病毒;对照组无特殊处理。感染后,各组动物隔离饲养。观察乳鼠的活动、饮食、体型、毛色和大便变化情况等,收集大便经胶体金法检测其中RV抗原。在接种后的3、5和8 d处死乳鼠,留取肝脏,免疫组化方法检测IFN-γ和IL-10水平。结果对照组2种细胞因子表达量较少,肠道内组IFN-γ水平在接种RV后的第3天明显增多,第3天至第8天缓慢减少;IL-10水平较正常组增高但整个过程未见明显变化。肠道外组IFN-γ水平与肠道内组比较差异无统计学意义;IL-10水平在感染第3天也明显增多,第3天至第8天有所减少但仍存在且高于正常组。结论 BALB/C乳鼠肠道内外感染RV动物模型建立成功。乳鼠肠道外感染早期及后期肝脏内细胞因子呈现出不同改变。所以在肠道外组,肝脏细胞因子平衡机制失衡,进一步阐明这种失衡可能是RV肠道外播散的重要机制。
Objective To establish the sucking mice model of intestinal and extraintestinal rotavirus infection,detect the level of INF-γ/IL-10 in the liver of sucking mice with intestinal and extraintestinal rotavirus infection,compare the change of cytokines between intestinal and extraintestinal rotavirus infection,as well as the difference in changes at different time points,and discuss the relationship between the disbalance of INF-γ/IL-10 immune cells with intestinal and extraintestinal rotavirus infection.Method 54 clean level BALB/C sucking mice of 3 to 5 days old were randomly divided into three groups,18 in each group.Extraintestinal group were infused 0.10 mL（1×10-5） TCID50 infectious titer of SA-11 virus through abdomen.Intestinal group were orally administered 0.10ml（1×10-5） TCID50 infectious titer of SA-11.Control group had no special treatment.After infection,the changes in the activities,diet,size,coat and stool of the mice were observed.Colloidal gold assay was used to detect the RV antigen in the stool.The IFN-γ/IL-10 levels were determined with immunohistochemistry at day 3,5 and 8 respectively.Result In the Intestinal group,the IFN-γ level increased in 3 days after inoculation of RV and then decreased slowly from day 3 to day 8;IL-10 level was higher than that of normal group without significant change.In the Extraintestinal group,the IFN-γ level showed no significant difference with that of Intestinal group;IL-10 level increased obviously in the third day and then decreased slightly from day 3 to day 8,but still higher than that of normal group.Conclusion BALB/C sucking mice model of intestinal and extraintestional RV infection was extablished successfully.There are changes of cytokines in the mice liver in the early and late stage of extraintestinal infection.This results in an imbalnace of liver cytokines,which might be one of the important mechanisms for extraintestinal spread of RV infection.
出处
《中国微生态学杂志》
CAS
CSCD
2011年第7期608-611,共4页
Chinese Journal of Microecology
基金
安徽省教育厅自然科学研究项目(2006KJ107C)
关键词
轮状病毒
感染
乳鼠
细胞因子
肝脏
Rotavirus； Infection； Suckling mice； Cytokine； Liver；
