摘要
目的 探讨恶性疟原虫ＤＮＡ 疫苗在小鼠体内的免疫反应。方法 将恶性疟原虫ＦＣＣ－ １／ＨＮ株有性期阶段的重组质粒ｐｃＤＮＡ３－ Ｐｆｓ２５ 经骨骼肌途径注射ＢＡＬＢ／ｃ小鼠，对注射部位的骨骼肌进行了预处理，即于注射前７ｄ 先在左后肢股四头肌注射０．５％ 盐酸布比卡因５０μｌ，注射深度为２ｍ ｍ 。观察免疫后不同时间点小鼠血清ＩｇＧ抗体滴度、脾淋巴细胞增殖反应、ＣＤ４＋ ／ＣＤ８＋ Ｔ细胞亚群比值和ＮＫ 细胞杀伤活性的变化。结果 １）重组质粒ｐｃＤＮＡ３－ Ｐｆｓ２５ 经肌肉注射途径免疫小鼠后，机体ＩｇＧ抗体滴度增高、特异性Ｔ淋巴细胞增殖反应增强、ＣＤ８＋ Ｔ细胞亚群比值增高以及ＮＫ细胞杀伤活性增强。２）肌肉注射为一有效的ＤＮＡ疫苗免疫途径。结论 采用编码有性期基因的重组质粒ｐｃＤＮＡ３－ Ｐｆｓ２５ 经骨骼肌注射途径免疫小鼠，能诱导机体有效的体液免疫、细胞免疫和ＮＫ细胞杀伤活性。
Aim\ To explore the immune response of mice vaccinated by DNA vaccine of Plasmodium falciparum.Method\ The recombinant pcDNA3-Pfs25 sexual stage from FCC-1/HN strain of Plasmodium falciparum was injected into BALB/c mice by intramuscular route.The injected sites of the muscle of left latter leg were treated with injecting 50μl of 0 5% bupivacaine to a depth of 2mm previously.The changes of IgG antibody value,the splenic lymphocyte proliferation,the CD4 +/CD8 + subgroups and NK cell killing activity in mice at different time were detected.Results\ 1)After injecting recombinant pcDNA3-Pfs25 into mice by intrmuscular route,it showed that IgG level in sera,the splenic T lymphocyte proliferation stimulated by specific antigen of Plasmodium falciparum,CD8 + T cell subgroup were all increased,and NK cell activity was improved. 2)Injecting DNA vaccine by intramuscular route is effective.Conclusion\ Mice vaccinated with recombinant pcDNA3-Pfs25 comtaining coding sexual stage gene of Plasmodium falciparum by intramuscular route could induce comparative humoral and cellular immune response,and NK cell acivity.This study has provided the experimental basis of immunology for DNA vaccine of Plasmodium falciparum.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1999年第6期36-39,共4页
Chinese Journal of Zoonoses
基金
中山医科大学科学基金
