摘要
目的 探讨原发性肝细胞癌（ＨＣＣ）中ＷＡＦ１／ＣＩＰ１ 基因ｍＲＮＡ 的表达与临床病理学指标的关系和意义。方法 采用半定量ＲＴＰＣＲ方法，检测ＷＡＦ１／ＣＩＰ１ 基因在３０ 例ＨＣＣ及其癌旁肝组织中的表达。结果 肝癌组织ＷＡＦ１／ＣＩＰ１ｍＲＮＡ 表达相对值低于其相应癌旁肝组织［（１ ．１０±０ ．４３）Ｕ 对（１．３１±０．４１）Ｕ，Ｐ＜０ ．０５］ ，有卫星灶形成的肝癌低于无卫星灶形成的肝癌［（０．８６ ±０．２９）Ｕ 对（１ ．３２ ±０．４４）Ｕ，Ｐ＜ ０．０１］ ，但其在ＨＣＣ的其他病理学指标中差异无显著性（ Ｐ＞０ ．０５） 。结论 ＷＡＦ１／ＣＩＰ１ 基因的表达异常可能参与ＨＣＣ的发生、发展，并与肝癌细胞的浸润和转移分子机制有关。
Objective\ To study the WAF1/CIP1 mRNA expression in human hepatocellular carcinoma (HCC) and its clinical pathological implication. Methods\ Using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR), the expression of WAF1/CIP1 mRNA in 30 HCC samples and their corresponding peritumor liver tissues was detected respectively. Results\ The expression level of WAF1/CIP1 mRNA in HCC samples was lower than that in peritumor liver tissues ( P <0.05). The expression level of WAF1/CIP1 mRNA in HCC samples with intrahepatic metastasis was lower than that in HCC samples without intrahepatic metastasis ( P <0.01). However, no significant difference in expression of WAF1/CIP1 mRNA in HCC samples was found in other clinical pathological parameters ( P >0.05). Conclusion\ The relative lower expression of WAF1/CIP1 mRNA in HCC might contribute to the carcinogenesis and progression of HCC and might be related to the mechanisms of infiltration and metastasis of HCC.\;
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1999年第6期487-488,共2页
Chinese Journal of Experimental Surgery
