摘要
目的 探讨Ｃｆｏｓ 基因在正常肝、硬化肝、肝癌组织中表达及肝组织癌变的机理。方法 收集肝癌切除标本和肝硬化门脉高压者肝活检组织各２０ 例，正常对照１０ 例，肝癌病例中乙肝阳性者１６例，肝硬化中肝炎后肝硬化１５ 例，血吸虫性肝硬化５ 例。应用ＳＰ免疫组化法计算其阳性率、阳性细胞指数、强阳性表达率。结果 肝癌和硬化肝组织的阳性率、阳性细胞指数均显著高于对照肝，而肝癌和肝硬化组却差异无显著性。阳性切片中，强阳性表达，肝癌显著高于肝硬化组。肝癌中乙肝阳性者阳性细胞指数与阴性者差异无显著性。肝硬化中肝炎性肝硬化和血吸虫性肝硬化亦差异无显著性。结论 Ｃｆｏｓ 作为一种早期应答基因，在肝组织由正常到硬化和癌变中有一个渐进性的量变过程。肝炎病毒和血吸虫都同样引起Ｃｆｏｓ 基因高表达，而后者正是肝组织癌变中的早期行为。
Objective To investigate the mechanism of liver carcinogenesis by comparing the different expression of C fos in cirrhosis and carcinoma. Methods\ Twenty specimens each for cirrhosis and carcinoma tissues and 10 specimens as control were collected and studied immunohistochemically using C fos antibody. The positive rate, index of positive cells and highly positive rate were calculated and compared statistically. Results\ There was no significant difference in positive rate between carcinoma group and cirrhosis group, while the positive rate in the both groups differed significantly from that in normal controls. For index of positive cells, no difference was found between HBsAg positive and negative patients with HCC or between posthepatitis and schistosome cirrhosis. Conclusion\ As an early immediate response gene, C fos has an inclination of increasing in the course of liver cirrhosis and carcinogenesis.\;
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1999年第6期500-501,共2页
Chinese Journal of Experimental Surgery
