摘要
目的：研究烧伤后大鼠心脏心肌细胞、血管内皮细胞（ＥＣ）的变化及早期补液对它们的影响。方法：应用了常规电镜技术及过氧化氢细胞化学方法。结果：烧伤后０．５ ｈ 血管ＥＣ肿胀，烧伤后４ ｈ 血管ＥＣ可向血管腔伸出较长突起，血管腔外有明显渗出，心肌细胞线粒体明显肿胀。过氧化氢细胞化学反应为血管ＥＣ血管腔面有较多致密沉积物。烧伤后８ ｈ 心肌细胞及血管ＥＣ损伤进一步加重。补液组烧伤后１，４，８ ｈ，心肌细胞及血管ＥＣ损伤明显减轻，过氧化氢细胞化学反应电子致密沉积物也较少。烧伤后８ ｈ 细胞损伤开始恢复，血管ＥＣ出现大量吞饮泡，血管外液体重吸收。结论：烧伤后心肌细胞及血管ＥＣ发生损伤，早期补液对损伤有明显减轻作用。
Objective: To study the changes of rat myocardial cells and neighbour capillary endothelial cells(EC) after burn and the effects of early fluid resuscitation.Methods: Routine electron microscopic technique and cytochemical methods of hydrogen peroxide were performed. Results: Thirty min after burn, EC swelling was observed. Four hours after burn, projections of EC were observed. Mitochondria in myocardial cells swelled evidently. There were evident oozes outside blood vessels. In hydrogen peroxide cytochemistry group, there were electron dense materials on the luminal face of the EC. Eight hours after burn,the myocardial cells and capillary EC were more severely injured. In 1,4,8 h groups with fluid resuscitation, the injury degree of myocardial cells and EC were lower than that in the groups without fluid resuscitation. In hydrogen peroxide cytochemistry group, the electron dense materials on the luminal face of the EC decreased. Furthermore at 8 h after burn, there were a lot of pinocytosis in EC. Oozes outside blood vessels were absorbed. Conclusion: After burn, rat myocardial cells and EC are injured. Early fluid resuscitation can reduce the injury.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1999年第10期780-782,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
关键词
烧伤
血管内皮细胞
补液
大鼠
心肌细胞
burns
fluid resuscitation
heart
capilary endothelial cells
