摘要
对盐生隐杆藻(Aphanothecehalophytica)胞外多糖的酸水解产物进行纸层析、薄层层析、气相层析分析。结果表明该多糖是由鼠李糖、岩藻糖、木糖、甘露糖、葡萄糖和葡糖醛酸组成的酸性杂多糖。红外光谱和核磁共振氢谱表明其糖苷键多数为β型, 少数为α型。高碘酸氧化和Sm ith 降解分析提示其糖苷键主要为1→3 位键合, 少部分为1→6和1→4 位键合。超离心分析测得相对分子质量为280 000。
The composition of exopolysaccharide produced by Aphanothece halophytica was identified by paper, thin layer and gas chromatography.It is a heteropolysaccharide comprised by rhamnose,fucose,xylose,mannose,glucose and glucuronic acid.IR and 1H NMR spectrum indicated that it contained more β glycosidic bond than α glycosidic bond.It showed that(1→3)linkages predominated over(1→4)and(1→6)linkages in EP by Periodate oxidation and Smith degradation.The molecular weight was determined to be 280 000 by ultracentrifugation. ABSTRACT The composition of exopolysaccharide produced by Aphanothece halophytica was identified by paper, thin layer and gas chromatography.It is a heteropolysaccharide comprised by rhamnose,fucose,xylose,mannose,glucose and glucuronic acid.IR and 1H NMR spectrum indicated that it contained more β glycosidic bond than α glycosidic bond.It showed that(1→3)linkages predominated over(1→4)and(1→6)linkages in EP by Periodate oxidation and Smith degradation.The molecular weight was determined to be 280 000 by ultracentrifugation. The composition of exopolysaccharide produced by Aphanothece halophytica was identified by paper, thin layer and gas chromatography.It is a heteropolysaccharide comprised by rhamnose,fucose,xylose,mannose,glucose and glucuronic acid.IR and 1H NMR spectrum indicated that it contained more β glycosidic bond than α glycosidic bond.It showed that(1→3)linkages predominated over(1→4)and(1→6)linkages in EP by Periodate oxidation and Smith degradation.The molecular weight was determined to be 280 000 by ultracentrifugation.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1999年第4期89-91,共3页
Journal of Nanjing Agricultural University
