摘要
目的:探讨银杏叶提取物(extract761 from Ginkgo giloba,EGb761)对黄曲霉毒素B1(aflatox B1,AFB1)致大鼠肝癌后相关蛋白表达的影响。方法:71只大鼠随机分为AFB1组(28只),AFB1+EGb761组(29只),空白对照组(14只)。第64周观察大鼠肝癌发生率,应用免疫组化法检测硫酸乙酰肝素类蛋白聚糖(MXR7)、环氧化酶(COX-2)以及细胞周期蛋白依赖性蛋白激酶抑制蛋白(p16)的表达情况。结果:AFB1组肝癌发生率明显高于AFB1+EGb761组(76%vs28%),χ2=10.602,P<0.05,对照组无肿瘤发生。AFB1+EGb761组MXR7蛋白含量明显低于AFB1组〔(1.488±1.178)vs(5.133±2.725)〕,F=18.638,P<0.001;AFB1+EGb761组p16蛋白含量明显高于AFB1组〔(2.456±1.014)vs(1.533±0.856)〕,F=24.091,P=0.03,AFB1组COX-2蛋白含量为3.305±0.566;AFB1+EGb761组为2.704±0.431,F=9.352,P=0.783。结论:银杏叶提取物下调大鼠肝组织MXR7蛋白的表达,提高p16蛋白的表达,有抑制AFB1致大鼠肝癌的发生作用。
OBJECTIVE： To investigate the effects of Ginkgo biloba extract （extract 761 from Ginkgo giloba, EGb761） on rela- tive protein expressions during hepatocarcinogenesis induced by af- latoxin B1 （AFB1） in rats. METHODS： The 71 rats were divided into three groups： Group AFB1 （28）, Group AFBlq-EGb761（29） and Group control（14）. The animals were sacrificed at the end of the experiment. The incidence of liver cancers in each group was stated. The expressions of heparan sulfate proteoglycan（MXR7）, Cycloxygenase-2（COX 2） and Cyclin dependent kinase inhibitor （P16） were observed by immunohistochemistry staining in above samples. RESULTS： The rate of hepatocellular carcinoma （HCC） in group AFB1 were significantly higher than group AFB1 ＋ EGb761（76% vs 28%,X^2=10.602,P〈0.05）. No tumor devel- oped in the control animals. The expression of MXR7 protein in group AFB1 ＋ EGb761 were significantly lower than group AFB1 （1.488±1. 178） vs （5. 183±2. 725）,（F=18. 638,P〈0.001）. p16 protein in group AFB1 ＋ EGb761 were significantly higher than group AFB1 （2. 456±1. 014） vs （1. 533±0. 856）, （F=24. 091,P=0.03）. The difference of COX-2 protein expression in group AFB1 and group AFB1 ＋ EGb761 had no significant （3.305±0. 566） vs （2. 704±0. 431）, （F=9. 352,P=0. 783）. CONCLUSION： Ginkgo biloba extract reduces MXR7 protein expression in rats and enhance the expression of p16 protein, and it may be inhibited the occurrence of liver cancer induced by AFB1.
出处
《中华肿瘤防治杂志》
CAS
2011年第11期847-850,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
李嘉诚基金会西部教育计划资助项目(教外港函[2001]33)
