Osteoinduction by Ca-P biomaterials implanted into the muscles of mice 被引量：2

Osteoinduction by Ca-P biomaterials implanted into the muscles of mice

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摘要 The osteoinduction of porous biphasic calcium phosphate ceramics (BCP) has been widely reported and documented,but little research has been performed on rodent animals,e.g.,mice.In this study,we report osteoinduction in a mouse model.Thirty mice were divided into two groups.BCP materials (Sample A) and control ceramics (Sample B) were implanted into the leg muscle,respectively.Five mice in each group were killed at 15,30,and 45 d after surgery.Sample A and Sample B were harvested and used for hematoxylin and eosin (HE) staining,immunohistochemistry (IHC) staining,and Alizarin Red S staining to check bone formation in the biomaterials.Histological analysis showed that no bone tissue was formed 15 d after implantation (0/5) in either of the two groups.Newly-formed bone tissues were observed in Sample A at 30 d (5/5) and 45 d (5/5) after implantation;the average amounts of newly-formed bone tissues were approximately 5.2% and 8.6%,respectively.However,we did not see any bone tissue in Sample B until 45 d after implantation.Bone-related molecular makers such as bone morphogenesis protein-2 (BMP-2),collagen type I,and osteopontin were detected by IHC staining in Sample A 30 d after implantation.In addition,the newly-formed bone was also confirmed by Alizarin Red S staining.Because this is the report of osteoinduction in the rodent animal on which all the biotechnologies were available,our results may contribute to further mechanism research. The osteoinduction of porous biphasic calcium phosphate ceramics （BCP） has been widely reported and documented,but little research has been performed on rodent animals,e.g.,mice.In this study,we report osteoinduction in a mouse model.Thirty mice were divided into two groups.BCP materials （Sample A） and control ceramics （Sample B） were implanted into the leg muscle,respectively.Five mice in each group were killed at 15,30,and 45 d after surgery.Sample A and Sample B were harvested and used for hematoxylin and eosin （HE） staining,immunohistochemistry （IHC） staining,and Alizarin Red S staining to check bone formation in the biomaterials.Histological analysis showed that no bone tissue was formed 15 d after implantation （0/5） in either of the two groups.Newly-formed bone tissues were observed in Sample A at 30 d （5/5） and 45 d （5/5） after implantation;the average amounts of newly-formed bone tissues were approximately 5.2% and 8.6%,respectively.However,we did not see any bone tissue in Sample B until 45 d after implantation.Bone-related molecular makers such as bone morphogenesis protein-2 （BMP-2）,collagen type I,and osteopontin were detected by IHC staining in Sample A 30 d after implantation.In addition,the newly-formed bone was also confirmed by Alizarin Red S staining.Because this is the report of osteoinduction in the rodent animal on which all the biotechnologies were available,our results may contribute to further mechanism research.

作者 Rui-na YANG Feng YE Li-jia CHENG Jin-jing WANG Xiao-feng LU Yu-jun SHI Hong-song FAN Xing-dong ZHANG Hong BU

机构地区 Key Laboratory of Transplant Engineering and lmmunology National Engineering Research Center for Biomaterials Department of Pathology

出处《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2011年第7期582-590,共9页 浙江大学学报（英文版）B辑（生物医学与生物技术）

基金 Project (No. 2005CB623901) supported by the National Basic Research Program (973) of China

关键词 OSTEOINDUCTION Hydroxyapatite/β-tricalcium phosphate Biphasic calcium phosphate OSTEOGENESIS Osteoinduction； Hydroxyapatite/-tricalcium 磷酸盐； Biphasic 钙磷酸盐；成骨

分类号 R687 [医药卫生—骨科学]

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