摘要
背景早产儿视网膜病变（ROP）的进展与多种新生血管调控因子有关，而基质细胞衍生因子·1（SDF．1）在氧诱导视网膜病变（OIR）动物模型视网膜中的表达及其作用机制尚不明确。目的对SDF-1在OIR小鼠模型视网膜中的表达进行定位和定量分析。方法应用随机数字表法将动物随机分组。将20只7日龄C57BL／6J幼鼠暴露在体积分数（75±2）％浓度的高氧状态下5d，随后在正常氧环境下5d，作为OIR组；另20只同日龄幼鼠作为正常对照组。通过免疫组织化学染色和实时荧光定量聚合酶链反应（real-timePCR）法观察视网膜的SDF-1的蛋白表达以及SDF-1mRNA的变化。结果OIR组12日龄小鼠的视网膜神经节细胞层可见SDF．1蛋白呈阳性表达；OIR组17习龄小鼠的神经节细胞层、内层视网膜的血管内皮细胞、新生血管内皮细胞可见SDF-1蛋白呈强阳性表达；正常对照组12日龄小鼠SDF-1蛋白和正常对照组17日龄小鼠SDF-1蛋白微弱表达于内层视网膜及视网膜血管附近。OIR组17日龄小鼠的SDF-1mRNA表达水平明显高于OIR组12日龄小鼠（P〈O．01）及正常对照组17日龄小鼠（P〈0．01）；OIR组12日龄小鼠SDF-1mRNA表达水平明显低于正常对照组12日龄小鼠（P〈0．05）。OIR组17日龄小鼠的SDF-1mRNA表达水平明显高于OIR组12日龄小鼠（t=8．072，P〈0．05）和正常对照组17日龄小鼠（t=10．026，P〈O．05）；OIR组12日龄小鼠SDF-1mRNA表达水平明显低于正常对照组12日龄小鼠（t=4．336，P〈0．05）。结论SDF-1在相对低氧状态下的视网膜中表达明显上调，因而可促进OIR的视网膜新生血管形成。
Background The development of retinopathy of prematurity （ROP） is associated with many regulatory cytokines related to neovascularization;however,the retinal expression and regulated mechanism of stromal cell-derived factor-1 （SDF-1） in mouse model of oxygen-induced retinopathy （OIR） remain uncertain. Objective This study was to investigate the expression of SDF-1 in retina of mouse model of OIR. Methods Forty 7-day-old C57BL/6J mice were divided into OIR group and control group. In OIR group,20 mice were exposed to 75% oxygen for 5 days and then to room air for 5 days. In control group,20 mice were raised in room air. The expression of SDF-1 in retina of mice was studied by immunochemistry and quantified by real time reverse transcriptase polymerase chain reaction （RT-PCR）. Results The positive immunohistochemical staining for SDF-1 was found mainly locating at the ganglion cell layer in 12-day-old mice of OIR group;the stronger positive immunohistochemical staining for SDF-1 was noted mainly locating at the ganglion cell layer, vascular endothelial cells of inner retina, neovascular endothelial cells in 17-day-old mice of OIR group; the delicate positive immunohistochemical staining for SDF-1 was both found mainly locating at the inner retina and being around the retinal vascular in 12-day-old mice of control group and 17-day-old mice of control group. The expression of SDF-1 mRNA in 17-day-old mice of OIR group was higher than that of 12-day-old mice of OIR group （t = 8. 072, P〈0.05 ） and 17-day-old mice of control group （ t = 10. 026, P〈0.05 ）, respectively. The expression of SDF-1 mRNA in 12-day-old mice of OIR group was lower than that of 12-day-old mice of control group （t =4. 336,P〈0. 05）. Conclusion SDF-I might improve the onset of retinal neovascularization of OIR.
出处
《中华实验眼科杂志》
CAS
CSCD
北大核心
2011年第7期625-629,共5页
Chinese Journal Of Experimental Ophthalmology
