摘要
探讨了测定条件对纤维素酶酶活力测定的影响．研究表明，测定条件对测定结果影响较大，因此进行纤维素酶酶活力测定时，必须对测定条件作出规定．纤维素酶是一种复合酶，底物纤维素不溶于水，底物的不饱和程度对纤维素酶酶活力测定的影响较大．底物的不饱和程度越高，所测得酶活力就越低．利用酶液稀释率、蛋白质浓度和酶活力之间的关系建立一校正方程，得出一种纤维素酶酶活力测定的校正方法．
In this paper,the effects of testing condition(for example,the different type of CMC,the different concentration of CMC,the size of filter paper and stirring rate) on the measurement of saccharifying cellulase activity were discussed.The data showed that these variables had great influence on the testing results. Therefore,the testing condition must be standardized. Cellulase is a complex enzyme composed of several components.Insoluble cellulase in water is resistant protein to the cellulase.Since the substrate level can't be high enough, it limits the conversion. The higher the enzyme concentration,the lower the cellulase activity.Therefore,in this paper,the relationship between enzyme dilution or protein concentration and reducing sugar was used to obtain a modifying equation for measuring cellulase activity. According to the equation,a modifying method for the measurement was studied.Furthermore,the average rate of enzyme hydrolysis was used to assay cellulase activity.
关键词
纤维素酶
酶活力
测定条件
cellulase,activity,testing condition
average rate of enzyme hydrolysis
modifying equation
