摘要
水产集约化养殖的迅速发展带来的抗生素环境问题已经受到各国学者的关注,尤其是环境残留抗生素对微生物耐药性的诱导和抗生素在食物中的残留,直接影响水生生态系统健康以及人类健康。应用固相萃取-高压液相色谱-串联质谱方法（SPE-LC-MS/MS）研究不同生物养殖水体（鱼塘、螃蟹池、蛏池、虾池、鸭池）中残留抗生素类型和质量浓度。结果表明,基于LC-MS/MS分析的固相萃取方法对滨海养殖水体中5类14种抗生素残留的检测具有较高的萃取效率,并且检测方法回收率在63%~124%之间;在典型滨海养殖区不同养殖水体中检出了3类7种抗生素（含磺胺类增效剂甲氧苄氨嘧啶）残留,最高质量浓度分别为诺氟沙星（3.54 ng.L-1,虾池）、氧氟沙星（14.8 ng.L-1,蛏池）、磺胺嘧啶（5.36 ng.L-1,鸭池）、磺胺二甲嘧啶（7.35 ng.L-1,虾池）、磺胺甲噁唑（18.5 ng.L-1,虾池）、氟甲砜霉素（5.00 ng.L-1,虾池）和甲氧苄氨嘧啶（40.2 ng.L-1,鸭池）,均低于已有的报道质量浓度水平。养殖水体中残留抗生素种类和质量浓度与养殖生物类型有关,螃蟹养殖水体抗生素残留种类（4种）最少,鱼和鸭养殖水体抗生素残留检出达6种,而检出的抗生素最高残留质量浓度主要来自于虾养殖水体。
The environmental problems caused by heavy use of prophylactic antibiotics in aquaculture have been increasingly receiving public concerns in the world.The induction of resistant microorganisms by antibiotics entering into environment and food-chains become a emerging problem for aquatic ecosystem health and human health.In this study,a solid-phase extraction-liquid chromatography-tandem mass spectrometry（SPE-LC-MS/MS） approach was used to determine antibiotics residuals in five aquaculture waters（fish,crab,razor clam,shrimp,and duck） in Jiulong River estuary of southeastern China.Results showed that the solid-phase extraction procedures had promised extraction efficiency for 14 residual antibiotics of 5 groups in aquaculture water samples.The overall recoveries of spiked 13C-labeled caffeine ranged between 63%~124%.In the investigated estuarine area,7 antibiotics from 3 groups were detected in the series of aquatic animal ponds.The highest concentrations of detected antibiotics were 3.54 ng？L-1 of norfloxacin（shrimp ponds）,14.8 ng？L-1 of ofloxacin（razor clam ponds）,5.36 ng？L-1 of sulfadiazine（duck ponds）,7.35 ng？L-1 of sulfamethazine（shrimp ponds）,18.5 ng？L-1 of sulfamethoxazole（shrimp ponds）,5.00 ng？L-1 of florfenicol（shrimp ponds）,and 40.2 ng？L-1 of trimethoprim（duck ponds）,respectively.But,the concentrations of residual antibiotics in these aquatic animal ponds were lower than other studies.The type and concentration of residual antibiotics varied between different aquacuture ponds,4 types of residual antibiotics were detected in crab ponds while 6 types in fish ponds and duck ponds.The highest concentrations of antibiotics were detected in shrimp ponds.
出处
《生态环境学报》
CSCD
北大核心
2011年第5期934-939,共6页
Ecology and Environmental Sciences
基金
中国科学院"百人计划"项目"城市湿地生态与环境"(A0815)
关键词
抗生素
水产养殖
滨海养殖区
养殖塘
antibiotics
aquaculture
estuarine aquaculture areas
aquaculture ponds
