摘要
目的比较不同的脱钙液对骨组织的脱钙能力的差异,探讨其对HE染色的影响。方法选取50例手术切除的新鲜骨标本,运用4种常用的脱钙液(14%硝酸、10%盐酸、20%甲盐酸和EDTA脱钙液)进行脱钙,比较其脱钙效果和对HE染色的影响。结果不同的脱钙液对骨组织的脱钙能力、HE染色效果影响有显著区别。结论 14%硝酸脱钙能力最强,用时间最短,染色的效果最差;10%盐酸对骨组织的损伤小,HE染色的效果最好,但用时较长;20%甲盐酸脱钙能力和HE染色效果都介于10%盐酸和EDTA脱钙液之间;EDTA脱钙液脱钙用时最长,对骨组织的损伤最小。
Objective To investigate the different ability of four different decalcifying solutions to decalcify and their influence of HE stain and immunohistochemistry. Methods 50 cases of fresh human bone example are selected. The bones had been decalcified with four kinds of decalcifying solution （including 14% nitric acid, 10% hydrochloric acid, 20% a hydrochloric acid and EDTA）. Results Different decalcifying solutions had the different decalcified ability and the influence of HE stain and immunohistochemistry. Conclusion 14% nitric acid can decalcify strongly and rapidly. But its deficiency is the infection of HE coloration and immunohistochemistry. 10% hydrochloric acid doesn＇t destroy the tissue and has the best influence for HE stain.The effect of 20% a hydrochloric acid and HE stain between 10% hydrochloric acid and EDTA decalcifying acid. EDTA can decalcify for a long,have the minimum damage for bone tissue.
出处
《中国实用医药》
2011年第19期27-28,共2页
China Practical Medicine
关键词
脱钙液
HE染色
骨组织
Decalcifying solution
HE stain
Bone tissue
