摘要
为了研究转录因子在玉米雌穗早期发育阶段的差异表达情况,探讨其生物学功能,利用Arizona大学的玉米芯片对未成熟玉米雌穂的早期关键发育阶段进行全基因组表达谱分析。数据标准化之后对差异基因中的转录因子家族进行超几何分析,挑选并分析差异明显的转录因子家族。结果表明,在玉米雌穗发育早期,有1 844个基因差异表达,有7个转录因子家族基因变化达到显著性差异,其中NAC类转录因子家族的变化最为显著,P值为5.6E-05。从生长锥伸长期到小穗分化期,该家族有9个基因上调表达,有6个成员下调表达。说明NAC类转录因子家族对玉米雌穗早期发育起到一定作用。
To study expression pattern of transcription factors during maize ear early developmental stage and supply some clues for their biological function,maize long oligonucleotide arrays made by Arizona University were used to analyze genome-wide expression of maize ears during the key developmental stage.Within-slide normalization and between-array normalization had been done before the different expressional genes were selected.Hyper geometric distribution was performed to analyze the transcription factor families.The expression of 1844 genes showed changes during the process of immature ear development,in which 7 transcription factor families were significantly differently expressed.Genes of the NAC protein family were differentially regulated in this phase and the P value reached 5.6E-05.Nine of them were up-regulated and six down-regulated from the growth cone elongation to the spikelet differentiation stage.It showed that the NAC transcription factors might play an important role during maize ear early developmental processes.
出处
《河南农业科学》
CSCD
北大核心
2011年第7期21-25,共5页
Journal of Henan Agricultural Sciences
基金
国家973计划(2006CB101700)
河北科技大学博士科研启动基金(QD200963)
河北科技大学校立科学研究基金项目(XL200765)
关键词
玉米
雌穂
发育
芯片
NAC类转录因子
Maize
Ear
Development
Microarray
NAC transcription factor
