摘要
目的研究肉苁蓉(Cistanche deserticolaMa)花粉原位萌发特性,初步判定其自交不亲和类型。方法应用荧光显微法观察肉苁蓉花粉粒的原位萌发、花粉管在柱头及花柱中的生长路径;比较不同授粉方式和授粉不同时间花粉萌发和花粉管生长特性,并通过胼胝质的产生部位判断自交不亲和类型。结果观察结果显示,授粉后40 min花粉管开始萌发,1 h后长度达一个花粉粒径,但自花花粉总量较少,仅萌发的部分留在柱头上。异花授粉10～12 h后,花粉管已经在花柱的中空处集成一束,远离柱头花粉管长度达8～15 mm;自花授粉48～72 h后花粉管基本停止伸长,测量长度仅为2.8～5.6 mm,仍处于柱头与花柱交界处,末端出现较长胼胝质样亮点。结论 肉苁蓉种群内存在自不亲和性,自交不亲和类型属配子体型;同花的花粉与柱头成熟期相同且空间分离较小,花粉容易落到自花的柱头上萌发但不能结实,这可能是肉苁蓉自然结实率较低的原因之一。
OBJECTIVE To study the characteristics of in situ pollen germination of C.deserticola and to judge the type of self-incompatibility of C.deserticola.METHODS Fluorescence microscopy was used to observe the in situ pollen germination and pollen tube growth in the stylar canal of C.deserticola.The characteristics in different phases after cross-pollination and self-pollination were compared,and the type of self-incompatibility was evaluated by generated location of callose in the pollentube.RESULTS Fluorescence microscopy showed that the pollen germinated 40 min after pollination,and the tubes were equally long as the diameter of pollen grain.But only parts of the pollens from self-pollination germinated and remained on the stigma.10-12 h after cross-pollination,the pollen tubes gathered to the stylar cannl and were 8-15 mm far from stigma.48-72 h after self-pollination,the pollen tubes almost ceased growing in the junction of the stigma and stylar,which were only 2.8-5.6 mm long.At the end of the tubes,the long bright callose spots were germinated.CONCLUSION The population of C.deserticola possesses the characteristic of self-incompatibility,and the type of self-incompatibility should be gametophytic type.The pollens and stigma from the same flower muture togother and have no much separation.Self-pollination is apt to emerge but with no seed setting.It may be one of the reasons for low seed setting percentage of C.deserticola.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2011年第14期1066-1069,共4页
Chinese Pharmaceutical Journal
基金
国家自然科学基金资助项目(30772727)
国家科技重大专项(2009ZX09308-002
2006BAI06A13-05)
关键词
肉苁蓉
荧光显微
花粉原位萌发
自交不亲和
Cistanche deserticola
fluorescence microscopy
pollen germination
self-incompatibility
