摘要
目的研究母鼠妊娠期和哺乳期铅暴露对仔鼠海马组织中肿瘤坏死因子-α(TNF-α)蛋白表达的影响。方法将60只健康成年清洁级妊娠昆明小鼠随机分为4组,分别为对照(蒸馏水)组和低剂量(0.3 g/L)、中剂量(1.0g/L)、高剂量(3.0 g/L)乙酸铅染毒组,每组10只。分别于妊娠期和哺乳期(即妊娠第1天至仔鼠出生21 d)采用自由饮水方式对母鼠进行染毒。每组取10只仔鼠,每窝取1只进行实验。分别于出生后第7、14、21天检测血铅和海马组织中的铅含量;出生后第21天采用Western blot方法检测海马组织中TNF-α的表达水平。结果不同剂量铅染毒仔鼠血铅和海马组织中铅含量均高于对照组,差异有统计学意义(P<0.05或P<0.01)。与对照组比较,中、高剂量铅染毒组仔鼠海马组织中TNF-α蛋白的表达水平均较高,差异有统计学意义(P<0.01)。经Pearson相关分析,仔鼠海马组织中TNF-α蛋白的表达水平与血铅、海马组织中铅含量均呈正相关(血铅:r=0.782,P<0.05;海马组织中铅含量:r=0.893,P<0.05)。结论妊娠期和哺乳期铅暴露可使仔鼠海马组织中TNF-α蛋白表达增高。
Objective To study the effects of lead exposure of pregnant mice on the expression of TNF-α protein of the offspring in mice. Methods Sixty pregnant Kunming mice were randomly divided into 4 groups, and treated with lead acetate at the doses of 0,0.3,1.0 and 3.0 g/L respectively, through drinking water, from the beginning of gestation and lasted until weaning （PND21）. The lead contents in blood and hippocampus of the offspring were determined 7,14 and 21 days after birth. At the 21st day, the expression of TNF-α protein in hippocampus of all the offspring in various dosages groups were determined by Western blot. Results The lead levels in blood and hippocampus of 7,14,21 d offspring in lead exposure groups were significantly higher compared with the control group （P〈0.05 or P〈0.01）. The results of Western Blot showed that the expression of TNF-α protein in hippocampus was higher in the moderate and high lead exposure group than control group （P〈0.01） .The analysis of correlation showed that the expression of TNF-α protein was positively related to lead content in blood and hippocampus （blood ：r=-0.782, P〈 0.05;hippocampus： r=0.893,P〈0.05）. Conclusion Maternal lead exposure may increase the accumulation of lead in blood and hippocampus and up-regulate the expression of TNF-α protein in hippocampus of pups.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2011年第7期592-594,共3页
Journal of Environment and Health
