摘要
首次将新合成的柱前衍生试剂6-氧-(N-琥珀酰亚胺乙酸酯)-9-(2′-甲氧羰基)荧光素(SAM F)用于巴氯芬(B aclofen)的衍生标记,并采用毛细管电泳分离-激光诱导荧光(CE-L IF)法分离检测衍生物。研究表明,在磷酸盐缓冲溶液(pH=7.5)介质中,30℃下10m in即可完成衍生反应。反应物在pH 5.6,35mm o.lL-1的磷酸盐缓冲溶液中12m in内达到分离,检出限为6×10-10m o.lL-1。本法用于血清中巴氯芬的分析测定,回收率为95.8%—101.0%。
A sensitive and efficient analytical method for separation and detection of baclofen in human serum by capillary electrophoresis coupled laser induced fluorescence（CE-LIF） method was established,6-oxy-（N-succinimidylacetate）-9-（2′-methoxycarbonyl） fluorescein（SAMF）,a new synthesized precolumn derivatization reagent,that was used as mark of derivatization for baclofen.The derivative reaction was completed in phosphate buffer（pH 7.5） at 30℃ for 10min.Optimal separation and detection were obtained in phosphate buffer（pH 5.6） of 35mmol·L^-1 for 12min and limit of detection of 6×10^-10mol·L^-1.The sensitive method was used to the determination of baclofen in serum samples,and recovery was from 95.8% to 101.0%.
出处
《光谱实验室》
CAS
CSCD
北大核心
2011年第4期1659-1662,共4页
Chinese Journal of Spectroscopy Laboratory
基金
中央高校基本科研业务费专项资金资助(11609702)
