摘要
利用超声-微波协同萃取技术从车前草中提取熊果酸与齐墩果酸,研究建立了反相高效液相色谱法快速测定车前草中熊果酸与齐墩果酸含量的方法。采用安捷仑C18柱(4.6mm×250mm,5μm)色谱柱,流动相为乙腈-0.5%乙酸铵(体积比80∶20),流速为0.8mL/m in,UV检测波长210nm。在该色谱条件下熊果酸与齐墩果酸能在12m in内达到基线分离。熊果酸在1.01—10.1μg范围内线性关系良好,r=0.9995,平均加标回收率为98.8%,相对标准偏差RSD为1.7%(n=5);齐墩果酸的峰面积与其质量浓度在0.39—3.9μg范围内呈良好的线性关系,r=0.9992,平均加标回收率为97.9%,RSD为1.3%(n=5)。该方法简便、快速、准确,可用于车前草中熊果酸与齐墩果酸的含量测定。
Ursolic acid and oleanolic acid were extracted from herba plantaginis by ultrasonic-microwave synergistic extraction technology,and a method for fast simultaneous the determination the contents of ursolic acid and oleanolic acid in herba plantaginis was established by reversed phase-high performance liquid chromatography（RP-HPLC）.The Agilent C18 column（4.6mm×250mm,5μm）was used with acetonitrile-0.5% ammonium acetate solution（80∶20,V/V）as the mobile phase at the flow rate of 0.8mL/min,and UV detection wavelength was at 210nm.The baselines of ursolic acid and oleanolic acid separated in 12 min under these chromatographic conditions.The calibration curves of ursolic acid and oleanolic acid were linear in the range of 1.01—10.1μg（r=0.9995） and 0.39—3.9μg（r=0.9992）,respectively.The average recoveries were 98.8%and 97.9%,and the RSD were 1.7%（n=5）and 1.3%（n=5）,respectively.The method is simple,quick and accurate,that can be used for simultaneous the determination of the contents of oleanolic acid and ursolic acid in herba plantaginis.
出处
《光谱实验室》
CAS
CSCD
北大核心
2011年第4期1922-1925,共4页
Chinese Journal of Spectroscopy Laboratory
