摘要
[目的]得到最佳的去除亮菌菌索糖复合物蛋白的方法。[方法]分别用Sevag法、酶法、三氯乙酸脱法、酶法与Sevag法结合法去除亮菌菌索糖复合物蛋白,筛选最佳脱蛋白方法。[结果]酶法与Sevag法结合脱蛋白的效果好,这时蛋白质含量仅为0.18%;酶法的最佳的条件:酶用量为0.5%,温度为40℃,水浴时间为0.5h,pH为6。[结论]酶法与Sevag法结合法是较好的脱蛋白法。
[Objective]To obtain the optimum method to remove protein form polysaccharide of Armillariella tabescens.[Method]Protein was removed from the polysaccharides of Armillariella tabescens respectively by Sevag method,enzyme method,trichloroacetic acid（TCA） method,and the combined method of enzyme and Sevag,and the best protein removal method was selected.[Result]The combined method of enzyme and Sevag was better than others with the contents of protein of only 0.18% ;the optimum conditions needed for this method were：0.5% of enzyme,40 ℃,0.5 h bath and pH 6.[Conclusion]The combined method of enzyme and Sevag was the best method for protein removal.
出处
《安徽农业科学》
CAS
北大核心
2011年第19期11443-11444,共2页
Journal of Anhui Agricultural Sciences
关键词
亮茵
多糖
脱蛋白
Armillariella tabescens
Polysaccharide
Protein removal
