摘要
[目的]筛选出具有抗真菌活性的粘细菌并对其进行种类鉴定。[方法]从全国23个省、自治区的200个腐殖质土样中分离纯化出237株粘细菌;将菌株在4种不同培养基中发酵培养,发酵产物通过甲醇提取得到代谢产物;以白色念珠菌为模型对次级代谢产物进行高通量筛选。[结果]复筛后得到1株编号为ZJ2的粘细菌,其次级代谢产物具有抗白色念珠菌的活性,对该菌株进行16SrDNA克隆并测序,结果表明ZJ2为橙色粘球菌(Myxococcus fulvus)。[结论]菌株ZJ2的次级代谢产物中,存在对白色念珠菌生长有显著抑制作用的生物活性物质,具有潜在的研究和药用价值。
[Objective]The paper was to screen myxobacterias with antifungal activity,and identify its species.[Method]237 myxobacterias were isolated and purified from 200 humus soil samples collected from 23 provinces in China.The strains were fermented in four different media,and the fermentation products were extracted by methanol to obtain metabolites.With Candida albicans as the model,the secondary metabolites were carried out high-throughput screening.[Result]A strain of myxobacteria numbered ZJ2 was obtained after secondary screening,its secondary metabolites had activities against Candida albicans.16SrDNA cloning and sequencing results showed that ZJ2 was Myxococcus fulvus.[Conclusion]The secondary metabolites of ZJ2 strain had bioactive substances with significant inhibition effect against the growth of C.albicans,which had potential study and medicinal value.
出处
《安徽农业科学》
CAS
北大核心
2011年第19期11485-11487,共3页
Journal of Anhui Agricultural Sciences
基金
陕西省科技厅农业公关项目"陕南特色生物资源利用与新产品开发"(2009K01-25)
陕西省教育厅产业化中试项目"绿色无公害微生物发酵饲料添加剂产业化"(2010JC22)
关键词
粘细菌
代谢产物
抗真菌活性
高通量筛选
Myxobacteria
Metabolites
Antiflmgal activity
High-throughput screening
