摘要
[目的]建立分光光度法测定中华鲟软骨中硫酸软骨素含量的方法。[方法]采用碱提-酶解法提取中华鲟软骨中硫酸软骨素,采用咔唑法,以D-葡萄糖醛酸为标准品,在硼砂-浓硫酸介质中经咔唑显色后,以蒸馏水为空白,于波长530nm处分别测定对照品溶液和供试品溶液的吸光度,按标准曲线计算葡萄糖醛酸含量,进而转换为硫酸软骨素含量。[结果]D-葡萄糖醛酸在10～50μg/ml范围内线性关系良好,回归方程为Y=0.015X-0.037(r=0.9997),3个不同浓度的加样回收率分别为100.87%、100.73%和101.29%(n=5),RSD值分别为2.74%、2.56%和2.22%。[结论]该方法快速,重现性好,可用作中华鲟软骨中硫酸软骨素含量的测定。
[Objective]The aim was to develop a spectrophotometry method for the determination of chondroitin sulfate from Chinese sturgeon cartilage.[Method]Chondroitin sulfate was extracted from Chinese sturgeon cartilage by the dilute alkali-enzyme hydrolysis method.The content of chondroitin sulfate from Chinese sturgeon cartilage was determined by the carbazole method and D-glucuronic acid was used as reference standard.A color reaction was made with carbazole using borax-sulfuric acid as medium.The absorbance was determined at 530 nm and the content of D-glucuronic acid was calculated by using the standard curve,and then converted into the content of chondroitin sulfate.The distilled water was taken as the blank control.[Result]The calibration curve was linear in the range of 10-50 μg/ml for D-glucuronic acid and the regression equiation was Y = 0.015X-0.037,r = 0.999 7.The average recoveries for three different concentrations were 100.87%,100.73% and 101.29%（n = 5）,and the relative standard deviations were 2.74%,2.56% and 2.22%,respectively.[Conclusion]The method was fast and had good reproducibility.It can be used to determine the content of chondroitin sulfate from Chinese sturgeon cartilage.
出处
《安徽农业科学》
CAS
北大核心
2011年第19期11597-11598,共2页
Journal of Anhui Agricultural Sciences
关键词
咔唑法
硫酸软骨素
D-葡萄糖醛酸
中华鲟软骨
含量测定
Carbazole method
Chondroitin sulfate
D-glucuronic acid
Chinese sturgeon cartilage
Content determination
