摘要
利用赤霉素、细胞分裂素、脱落酸、乙烯利和吲哚乙酸分别涂抹板栗短雄花序,结果显示仅赤霉素能够抑制短雄花序顶端的细胞程序性死亡,并在一定程度上恢复短雄花序的伸长生长。通过RT-PCR扩增方法,从板栗短雄花序芽变和正常雄花序cDNA中克隆出赤霉素受体基因GID1部分序列。测序及序列分析结果表明:克隆的cDNA片段为786 bp,所推导的氨基酸序列与杨毛果GID1氨基酸序列有91%的同源性,与陆地棉、蓖麻GID1均有85%的氨基酸序列同源性。经氨基酸序列功能分析,推测该GID1序列是板栗有编码功能的赤霉素受体基因,命名为CmGID1(GenBank登录号为HQ651231)。实时荧光定量PCR结果显示,从花序萌发至花药萌发前期,除其中一个时期(5月23日)外,芽变短雄花序CmGID1的表达量均高于正常雄花序;在花序的快速生长期,芽变短雄花序CmGID1表达量显著高于正常雄花序(P<0.01);且赤霉素处理后恢复生长的短雄花序CmGID1表达量显著降低(P<0.01)。综合试验结果,初步揭示了该短雄花序为一个赤霉素缺陷型突变体。
GAs,CTK,ABA,Ethephon and IAA were used separately in feeding experiments,the result showed only the application of GAs can inhibit programmed cell death（PCD）in the top of short-male catkin and recover elongation growth to some extent.On the other hand,the partial cDNA sequnences encoding gibberellins receptor GID1 was isolated from the normal and mutant catkin of the Castanea mollissima by reverse transcription polymerse chain reaction（RT-PCR）.The results showed that the GID1 partial cDNA is 786 bp,putative amino acids have 91% similarity with GID1 in Populus trichocarpa and 85% in Ricinus communis and Gossypium hirsutum.According to the function analysis of putative animal acid sequnence,the GID1 is a functional GAs receptor gene in Castanea mollissima,and named CmGID1（accession numbers HQ651231）.Real-time PCR indicated that from primordia formation phase to early germina-tion of anther,the expressions of CmGID1 gene in mutant catkins are higher than that in normal catkins except the phase of May 23.Moreover,in the fast-growing phase,the expression of CmGID1 gene in mutant catkins are significantly higher than that in normal catkins（P 0.01）,while in growth recovered short male catkin treated after GAs,the expression of CmGID1 gene significantly decreased（P 0.01）.In conclusion,it was preliminarily revealed that the short-catkin chestnut bud mutation is a GAs-deficient mutant.
出处
《园艺学报》
CAS
CSCD
北大核心
2011年第7期1251-1258,共8页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30872039)
北京市自然科学基金项目(6082004)
北京市科技新星计划项目(2007B031)
