摘要
目的:测定狗娃花(根部)多糖的含量。方法:以葡萄糖为标准品,用苯酚-硫酸法测定狗娃花(根部)多糖的含量。结果:葡萄糖标准品在498.5nm有最大吸收,回归方程为A=16.9715c-0.08919(r=0.9995),精密度(RSD)为1.536%,多糖的稳定性(RSD)为0.223%,加样回收率为98.736%,狗娃花(根部)中多糖含量为0.0168%。结论:此测定方法为化学法,所需仪器设备简单,操作方便,适用于狗娃花(根部)多糖含量的测定,也为其他多糖含量的测定提供了参考。
Objective：To determine polysaccharide content of Heteropappus altaicus（the root）.Method：By the phenol-sulfuric acid method,with glucose as standard substance,was used to determine polysaccharide content of Heteropappus altaicus.Results：The standard glucose at 498.5nm has the maximum absorption volume,the return equation is A=16.9715c-0.08919（r=0.9995）,precision（RSD） is 1.536%,the stability of polysaccharide（RSD） is 0.223%,and recovery percentage is 98.736%,the polysaccharide content of Heteropappus altaicus（the root） is 0.0168%.Conclusion：The determination is a chemical method,the apparatus required are simple,easy to operate,which is suitable for polysaccharide content determination of Heteropappus altaicus（the root）,in addition it provides a reference for other polysaccharide content determination.
出处
《内蒙古医学院学报》
2011年第3期182-185,共4页
Acta Academiae Medicinae Neimongol
基金
内蒙古自治区自然科学基金(2010MS1206)
关键词
狗娃花
多糖
苯酚-硫酸法
Heteropappus altaicus
polysaccharide
phenol-sulfuric acid method
