摘要
目的:探讨6种常见的乙肝病毒(HBV)感染模式与乙肝病毒DNA(HBV-DNA)病毒载量之间的关系。方法:运用酶联免疫吸附法(ELISA)、实时荧光定量聚合酶链反应(FQ-PCR)技术对1022例乙肝患者进行血清标志物(两对半)及HBV-DNA含量检测。结果:血清学检测乙肝表面抗原(HBsAg)+乙肝病毒e抗原(HBeAg)+乙肝病毒核心抗体(HBcAb)阳性(大三阳)患者HBV-DNA阳性率为94.1%明显高于其他模式,且其病毒载量显著高于其他组。HBsAg+乙肝病毒e抗体(HBeAb)+HBcAb(小三阳)阳性检出率也较高(阳性率54.3%)。结论:HBeAg是反映HBV复制活跃的可靠指标,HBV-DNA与HBeAg的存在明显正相关;HBV-DNA病毒载量可真实反映HBV感染、复制及病程变化,对乙型肝炎临床诊断及治疗均有较大的指导意义。
Objective： To investigate the 6 common hepatitis B virus （HBV） and hepatitis B virus infection model DNA （HBV-DNA） the relationship between viral load. Methods：Using enzyme-linked immanosorbent assay （ELISA）, real-time fluorescence quantitative polymerase chain reaction （FQ-PCR） technique in patients with 1022 cases of hepatitis B serum markers （two half）, and HBV-DNA content measurements.Results：The serological detection of hepatitis B surface antigen （IfBsAg） ＋ Hepatitis B virus e antigen （HBeAg） ＋ Hepatitis B virus core antibody （HBcAb）-positive （HBeAg） in patients with HBV-DNA positive rate was 94.1%, significantly higher than other models, and The viral load was significantly higher than other groups. HBsAg ＋ hepatitis B virus e antibody （HBeAb） ＋ HBcAb （three positive） and higher positive rate （positive rate 54.3%）. Conclusions： HBeAg reflects a reliable indicator of active replication ofHBV, HBV-DNA and the presence of HBoAg was positive; HBV-DNA viral load could be a true reflection of HBV infection, replication and course changes, clinical diagnosis and treatment of hepatitis B have greater significance.
出处
《中国医药导刊》
2011年第6期1050-1051,共2页
Chinese Journal of Medicinal Guide
