摘要
目的:观察阴道乳杆菌菌株在体外实验中对假丝酵母菌生长的抑制作用。方法:分离健康妇女阴道中常见的乳杆菌和外阴、阴道假丝酵母菌病(vulvovaginal candidiasis,VVC)患者阴道中的常见假丝酵母菌,采用96孔板混合生长抑制法观察处于生长对数期的乳杆菌对假丝酵母菌生长的抑制作用。结果:23例健康妇女阴道分泌物中分离出3种乳杆菌:嗜酸乳杆菌(La)、干酪乳杆菌(Lca)和卷曲乳杆菌(Lcr),15例VVC初发患者阴道分泌物中分离出3种假丝酵母菌:白色假丝酵母菌(Ca)、热带假丝酵母菌(Ct)和克柔假丝酵母菌(Ck)。各种乳杆菌均表现出对假丝酵母菌生长的抑制能力,La对各种假丝酵母菌的抑制能力均优于Lcr;与Lca相比,La对Ct的抑制更为明显;产H2O2的Lcr与不产H2O2的Lca对假丝酵母菌的抑制能力无显著差异。结论:各种乳杆菌表现出对假丝酵母菌生长的不同抑制能力,La对研究的3种假丝酵母菌的抑制能力均优于Lcr;与Lca相比,La的唯一优势体现在对Ct的抑制上;乳杆菌对假丝酵母菌的抑制可能不仅仅与产生H2O2能力有关。
Objective： To observe the growth inhibition effects of Lactobacillus strains on strains of Candida in vitro.Methods： To separate common Lactobacillus strains from healthy women,and common Candida strains from patients with incipient vulvovaginal candidiasis（VVC）,and then to observe the growth inhibition of Lactobacillus strains within the vigorous growing period on strains of Candida by mixed growing in 96-holes plate.Results： Three isolates of Lactobacillus strains from vaginal secretions of healthy women were obtained： Lactobacillus acidophilus,Lactobacillus casei,Lactobacillus crispatus,and 3 isolates of Candida from women with incipient VVC： Candida albican,Candida tropicalis and Candida krusei.Lactobacilli in the experiment all demonstrated the growth inhibition effects on Candida to certain extent.Lactobacillus acidophilas was superior to Lactobacillus crispatus in the inhibition of all the Candidas in the experiment.Compared with Lactobacillus casei,Lactobacillus acidophilas could significantly inhibit the Candida tropicalis.There was not significant difference in the inhibition of candidas between Lactobacillus crispatus and Lactobacillus casei.While the Lactobacillus crispatus produces H2O2 and the Lactobacillus casei does not.Conclusion： Different ability to inhibit the vaginal Candida.Lactobacillus acidophilas had higher ability in the inhibition of various strains of Candida compared with Lactobacillus crispatus.Compared with Lactobacillus casei,the only advantage of Lactobacillus acidophilus is the high inhibition on Candida tropicalis.The inhibition ability of Lactobacillus strains on Candida may be not merely related to the production capacity of H2O2.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2011年第7期444-448,共5页
Reproduction and Contraception
