摘要
目的:建立不同形式大鼠游泳运动模型,探讨运动后大鼠孤束核内c-fos的表达及其时效性分布规律。方法:将55只正常雄性SD大鼠随机分为对照组(5只)、持续游泳运动组(25只)和间歇游泳运动组(25只)。持续游泳运动组和间歇游泳运动组通过6周游泳训练建立运动模型,在运动结束后0、0.5、1、2、4 h等不同时间点取材,冰冻切片,SABC法行免疫组织化学染色,计数孤束核内c-fos阳性神经元的数目,并进行统计学分析。结果:持续游泳运动组大鼠c-fos阳性神经元数在运动结束后逐渐增加,至0.5 h达高峰,之后回落,2 h时达运动后的低点,而后重新升高。间歇游泳运动组大鼠运动后孤束核内c-fos阳性神经元数显著增高至峰值,之后下降,至0.5 h后重新缓慢升高至接近于峰值水平,并维持于这一较高的表达水平。结论:孤束核在大鼠不同游泳运动后机体的调节作用中起重要作用。
Objective： To explore the expression,distribution and its rhythem of c-fos in nucleus tractus solitarius（NTS） of rats that treating with different kinds of swimming exercises.Methods： Fifty-five SD rats were randomly divided into control group（n=5）,endurance swimming group（n=25） and interval swimming group（n=25）.The rats in the latter two groups were trained for 6 weeks.Brains were removed at 0,0.5,1,2,4 h after swimming and cut into the sections,and immunohistochemical staining by SABC method was used in present study.We then count the nubmer of the c-fos immunopositive neurons,and made the statistic analysis.Results： In the endurance swimming group the number of c-fos immunopositive neurons increased gradually and reached peak at 0.5 h,and then decreased at 2 h.Then it increased again.Moreover,the number of c-fos immunopositive neurons in NTS of rats in interval swimming group reached the highest level,then turn down,and then increased to the level near the peak after 0.5 h and mainteined.Conclusion： The NTS plays an important role in the physical regulation after swimming exercise.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2011年第4期395-398,共4页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(30671019)
山西医科大学科技创新基金(01200803)
关键词
C-FOS
孤束核
游泳运动
大鼠
c-fos； nucleus tractus solitarius； swimming exercise； rat
