摘要
目的：分析ＡＣＥ 基因第１６ 内含子Ｉ／Ｄ 在内蒙古地区蒙古族人群中分布的多态性。方法：ＤＮＡ 提取自９６ 名健康蒙古族，应用ＡＣＥ 基因内含子１６ 多态部位侧翼的前体进行ＰＣＲ 反应。等位基因在琼脂胶上电泳，紫外灯下观察。结果：蒙古族ＤＤ、ＤＩ和ＩＩ基因型的频率是０－５１、０－２６ 和０－２３ 。等位基因Ｄ 和Ｉ频率是０－６４ 和０－３６ 。结论：内蒙古地区蒙古族ＤＤ、ＤＩ和ＩＩ基因型频率和汉族人群之间有显著性差异。蒙古族人群中ＡＣＥ 等位基因和基因型的分布；Ｄ 等位基因比Ｉ等位基因频率高，ＤＤ 基因型是最普遍的。
Objective:To analyse the insertion/deletion polymorphism of ACE gene in Mongolian.Methods:The fragment located at intron 16 of ACE gene were examined by PCR. Results:The frequencies of DD, DI and II genotype were 0.51, 0.26 and 0.23 in Mongolian.Allele frequencies for D and I were 0.64 and 0 36.Conclusion:The genotype of Mongolian were compared with the genotype of Hans, showing a significant difference between these two nationalities. The ACE allele and genotype frequencies are reported for Inner Mongolia Mongolian. The D allele occurs more frequently than I allele and DD genotype is the most common.
出处
《内蒙古医学院学报》
1999年第4期215-217,共3页
Acta Academiae Medicinae Neimongol
基金
内蒙古自然科学基金
