摘要
目的：研究乳腺良恶性病变核仁组织区嗜银蛋白和细胞形态定量。方法：利用改良的ＡｇＮＯＲ 染色和传统的ＨＥ染色测定乳腺良恶性病变ＡｇＮＯＲ 颗粒面积、数目和细胞形态定量。结果：（１） 细胞面积、核面积、核质比值、细胞周长、ＡｇＮＯＲ 颗粒面积及颗粒数目在乳腺良恶性病变之间存在显著差别（ Ｐ＜ ０－０００５） ；（２） 细胞面积、核面积及颗粒面积在Ⅰ级乳腺癌与Ⅱ级和Ⅲ级乳腺癌之间有显著差别（ Ｐ＜ ０－０１） ，核质比值在Ⅰ级与Ⅲ级之间有显著差别（ Ｐ＜０－０１） ；（３） 颗粒数目在Ⅲ级乳腺癌与Ⅱ级、Ⅰ级乳腺癌有显著差别（Ｐ＜ ０－０５ 和Ｐ＜ ０－００５） ；（４） 细胞形态各参数与颗粒面积和颗粒数目高度相关。结论：ＡｇＮＯＲ 和细胞形态定量可作为乳腺良恶性病变鉴别、乳腺癌分级的客观指标。
Objective:To study nuclear organizer region-associated protein and cytomorphology in breast benign and malignant lesions. Methods: Improved AgNOR and traditional HE staining were used to measure granular number and area of AgNOR and cytomorphological parameters. Results: (1)There were highly significant discriminitions of cellular area,nuclear area,nucleus/cytoplasm,cellular perimeter and granular area and number between breast benign and malignant lesions(P<0 0005). (2)Comparing with grade Ⅰ,cellular area,nuclear area and granular area and number were higher in grade Ⅱ and Ⅲ. But nucleus/cytoplasm was higher in grade Ⅲ than grade Ⅰ. (3)There existed significant difference of granular number between grade Ⅲ and Ⅱ, grade Ⅲ and Ⅰ. (4) All cytomorphological parameters were correlated with granular number and area. Conclusion:Cytomorphological parameters and granular area and number can be taken for objective signs for discriminiting breast benign and malignant lesions and grading of breast carcinoma.
出处
《内蒙古医学院学报》
1999年第4期229-231,共3页
Acta Academiae Medicinae Neimongol
