摘要
目的:通过检测核干细胞因子(Nucleostemin,NS)基因在多种肿瘤细胞中的差异表达情况,探讨NS基因与肿瘤细胞增殖调控的相关性。方法:体外培养子宫颈癌细胞、乳腺癌细胞、乳腺导管癌细胞、肝癌细胞(SMCC-7721)、肝癌细胞(HpeG2)与正常肝细胞,提取对数生长期肿瘤细胞总RNA,以RT-PCR方法检测NS在mRNA水平的差异表达;提取肿瘤细胞的总蛋白,以Western blot方法检测NS在翻译水平的差异表达。结果:肿瘤细胞中NS基因均有较高水平的表达,尤其以乳腺癌细胞、乳腺导管癌细胞、肝癌细胞(SMCC-7721)与肝癌细胞(HepG2)中NS基因表达量为最高,而正常肝细胞未检测到NS的表达。结论:NS在肿瘤细胞中的高表达具有普遍性,而在终末分化的细胞中不表达,判断NS基因参与了肿瘤细胞的增殖调控,其为肿瘤的基因治疗和基因诊断提供了一个新的途径和指标。
Objective：To detect the Nucleostemin（NS） gene expression in some tumor cells,and study the effect of NS in cell proliferation.Methods：Cervical cancer cells,breast cancer cells,ductal carcinoma,hepatoma cells（SMCC7721）,hepatoma cells（HpeG2） and normal liver cells were cultured in vitro.Cells in logarithmic growth phase were collected and total RNA was extracted from 6 kinds of cultured tumor lines.The NS differential expression level was measured by RT-PCR and western blotting.Results：All tumor cells in a high level of NS gene expression,especially SKRB-3 breast cancer cells,breast ductal carcinoma T47-D cells,hepatoma SMCC-7721 and HepG2 cells had the highest expression of NS gene,and no NS expression was detected in normal liver cells.Conclusions：NS is highly expressed among all kind of tumor cells is universality,and no expression in terminally differentiated normal liver cells.NS genes could involve in tumor cell proliferation regulation,which provided new method and target for cancer gene therapy and gene diagnosis.
出处
《蚌埠医学院学报》
CAS
2011年第7期669-672,共4页
Journal of Bengbu Medical College
基金
安徽省教育厅自然科学研究资助项目(KJ2009B255Z)
安徽省自然科学基金资助项目(10040606Q43)
安徽省高校省级优秀青年人才基金重点资助项目(2010SQRL123ZD)
关键词
肿瘤细胞
核干细胞因子
增殖调控
差异表达
tumor cells
Nucleostemin
proliferation regulation
differential expression
