摘要
对色氨酸酶基因工程菌 ＷＷ－ １１进行固定化及培养条件研究，为工业化生产 Ｌ－色氨酸奠定基础。方法；通过色氨酸酶活力测定，考察三种固定化材料及温度、 ｐＨ、单价阳离子和乙醇对固定化 ＷＷ－ １１色氨酸酶活力的影响。结果：以聚乙烯醇作为ＷＷ－１１的固定化载体，其活力回收为６０．９％。固定化ＷＷ－１１色氨酸酶降解反应最适ｐＨ为９．０、最适温度为５０℃；固定化ＷＷ－１１色氨酸酶合成反应最适ｐＨ为７．５、最适温度为４５℃。Ｋ＋、ＮＨ＋对固定化工程菌色氨酸酶有明显激活作用，而Ｎａ＋则有一定的抑制作用。结论：固定化工程菌色氨酸酶对温度、乙醇等的稳定性比游离菌有显著提高。
Purpose: Immobilization and culture conditions of tryptophanase genetic engineering strain WW-11 were studied to provide the basis for the industrial production of L-tryptophan. Methods: Effects of three immobilized materials, temperature, pH, monovalent canons and alcohol on the activity of tryptophanase of immobilized WW-11 were observed by enzyme assay. Results: PVA served as immobilized carrier of NO. WW-11, and its activity recovery of tryptophanase was 60. 9%. The optimum pH and temperature of degradative reaction by tryptophanase of immobilized WW-11 were 9. 0 and 50℃ .The optimum pH and temperature of synthetic reaction by tryptophanase of immobilled WW-11 Were 7. 5 and 45℃. Tryptophanase of immobilized engineering strian was activated by K+ and NH4+, and inhibited by Na+. Conclusion: The stability of tryptophanase of immobilized engineering strain by temperature and alcohol was superior to that of free strain.
出处
《中国生化药物杂志》
CAS
CSCD
1999年第6期271-274,共4页
Chinese Journal of Biochemical Pharmaceutics
关键词
色氨酸酶
基因工程菌
固定化
聚乙烯醇
酶活
Tryptophanase genetic engineering stain, Immobilization, PVA, Enzyme activity
