摘要
The paper reports a novel amperometric biosensor for catechol based on immobilization of a highly sensitive horseradish peroxidase by affinity interactions on metal chelate-functionalized agarose/carbon nanotubes composites. Metal chelate affinity takes advantage of the affinity of Ni2＋ ions to bind strongly and reversibly to histidine or cysteine tails found on the surface of the horseradish peroxidase. Thus, enzymes with such residues in their molecules can be easily attached to functionalized aga- rose/carbon nanotubes composites support containing a nickel chelate. Linear sweep voltammograms and amperometry are used to study the proposed electrochemical biosensor. Catechol is determined by direct reduction of biocatalytically liberated quinone species at -0.05 V （vs. SCE）. The effect ofpH, applied electrode potential and the concentration of H2O2 on the sensitivity of the biosensor has been investigated. The performance of the proposed biosensor is tested using four different phenolic compounds, showing very high sensitivity, in particular, the linearity of cateehol is observed from 2.0 × 10-8 to 1.05×10-5 M with a detection limit of 5.0×10-9 M.
The paper reports a novel amperometric biosensor for catechol based on immobilization of a highly sensitive horseradish peroxidase by affinity interactions on metal chelate-functionalized agarose/carbon nanotubes composites. Metal chelate affinity takes advantage of the affinity of Ni2+ ions to bind strongly and reversibly to histidine or cysteine tails found on the surface of the horseradish peroxidase. Thus, enzymes with such residues in their molecules can be easily attached to functionalized agarose/carbon nanotubes composites support containing a nickel chelate. Linear sweep voltammograms and amperometry are used to study the proposed electrochemical biosensor. Catechol is determined by direct reduction of biocatalytically liberated quinone species at -0.05 V (vs. SCE). The effect of pH, applied electrode potential and the concentration of H2O2 on the sensitivity of the biosensor has been investigated. The performance of the proposed biosensor is tested using four different phenolic compounds, showing very high sensitivity, in particular, the linearity of catechol is observed from 2.0 × 10-8 to 1.05 × 10-5 M with a detection limit of 5.0 × 10-9 M.
基金
supported by the National Outstanding Youth Foundations of China (50725825)
National Basic Research Program of China (2007CB310501 & 2011CB935704)
National Natural Science Foundation of China (50908113)
the Natural Science Foundation of Jiangxi Province (2008GZH0008)
the Youth Foundation of Jiangxi Provincial Department of Education (GJJ09483)
