摘要
目的 探讨叶酸对去卵巢大鼠骨质疏松的保护作用.方法 40只3月龄雌性SD大鼠随机分为5组：假手术组、去卵巢组、乙烯雌酚组（乙烯雌酚0.03 mg·kg-1·d-1）、低剂量叶酸组（叶酸5 mg·kg-1·d-1）、高剂量叶酸组（叶酸20 mg·kg-1·d-1）.各组大鼠于术后1周开始灌胃给药,治疗10周,假手术组和去卵巢组给予溶媒灌胃.测定大鼠血浆总同型半胱氨酸（tHcy）浓度,骨匀浆中碱性磷酸酶（ALP）和抗酒石酸酸性磷酸酶（TRACP）的水平;取右股骨和腰5椎体进行骨密度和骨生物力学测定,取腰6椎体和左股骨制备HE切片,观察骨组织的形态学变化.结果 与假手术组比较,去卵巢大鼠血浆tHcy浓度明显升高,腰椎和股骨骨密度显著减低（均P＜0.01）,血浆tHcy浓度与腰椎骨密度呈负相关（r=-0.359,P=0.040）.叶酸显著降低去卵巢大鼠血浆tHcy浓度（均P＜0.01）.大剂量叶酸显著增加去卵巢大鼠骨匀浆ALP水平,降低TRACP水平,增加腰椎和股骨骨密度（均P＜0.01）,改善腰椎和股骨的生物力学性能.结论 去卵巢大鼠存在高同型半胱氨酸血症,高同型半胱氨酸参与了去卵巢大鼠骨质疏松的发生.叶酸对去卵巢大鼠骨质疏松具有保护作用,其机制可能与改善同型半胱氨酸的代谢作用有关.
Objective To investigate the protective effect of folic acid（FA） on osteoporosis in ovariectomized（OVX） rats.Methods Forty three-month-old female SD Rats were divided into 5 groups, sham operation group, OVX group, diethylstilbestrol group（0.03mg·kg-1·d-1）,low dose FA Group （5 mg·kg-1·d-1）,and high dose FA group （20 mg·kg-1·d-1）.Gastric gavage in each group was started from one week after being ovariectomized and lasted 10 weeks. Sham operation group and OVX group were treated with solvent. The rats were sacrificed at the end of 10th week after treatment. The total homocysteine（tHcy） in plasma, alkaline phosphatase（ALP）, and tartrate-resistant acid phosphatase（TRACP） activity of bone homogenates were measured. The bone mineral density（BMD） and bone biomechanics were determined using L5 vertebrae and right femur. The bone tissue slices were made with L6 vertebrae and left femur and HE stained, and then the histomorphology was observed. Results Compared with sham operation group, plasma tHcy level was significantly increased（P〈0.01）, BMD of lumbar vertebrae and femur was remarkedly decreased in OVX group（all P〈0.01）. Plasma tHcy concentration was negatively correlated with lumbar BMD（r=-0.359, P=0.040）. Plasma tHcy level in both groups treated with folic acid was significantly reduced（all P〈0.01）. The ALP concentration in bone homogenates was higher, the TRACP concentration in bone homogenates was lower, and BMD and bone biomechanics of lumbar vertebrae and femur were increased in high dose FA group than those in OVX group（all P 〈0.01）. Conclusions In OVX rats hyperhomocysteinemia existed and was involved in the development of osteoporosis. Folic acid could protect OVX rats from osteoporosis, due probably to improved homocysteine metabolism.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2011年第7期594-598,共5页
Chinese Journal of Endocrinology and Metabolism
