摘要
以ME3+BK+100 g/L蔗糖+150 g/L PEG4000作为培养基,把桔梗新鲜花粉在不同的温度下进行培养,结果表明,30℃培养1.5 h为花粉萌发和花粉管生长的最佳培养条件。把桔梗花粉贮藏在不同的温度下,结果显示:低温贮藏可明显延长花粉的寿命,常温下花粉仅能贮藏4 d左右,但在10、4、-20℃下贮藏,花粉寿命可分别达60、90、150 d以上;随着贮藏时间的延长,花粉的授粉结实率均呈下降趋势,但低温贮藏可明显减缓结实率下降的速度。
Pollens of Platycodon grandiflorum were cultured in the culture medium of ME3＋BK＋10% suerose＋150 g/L PEG （pHS.8）. The results showed that the pollen germination were best at 30 ℃ for 1.5 h. Pollens of Platycodon grandiflorum were stored under different temperatures. The result showed that life of pollen at being stored under low temperature was prolonged significantly. The life span on pollen stored at 10 ℃, 4 ℃ and -20℃ in icebox was 60 d, 90 d and 150 d, respectively, while that for ambient temperature was about 4 d. Seeds setting of pollen after stored under different temperature was decreased with the storagetime extended, and seeds setting of pollen under ambient temperature storage was lower than those under low storage temperature.
出处
《热带作物学报》
CSCD
2011年第5期956-959,共4页
Chinese Journal of Tropical Crops
基金
陕西省科学技术发展计划项目(2008k16-02)
陕西省教育厅专项科研项目(2010JK252)
关键词
桔梗花粉
贮藏温度
生活力
结实率
Platycodon grandiflorum
Pollen
Geramination in vitro
Storage
Seeds setting
