摘要
目的研究人工虎骨粉对大鼠骨折愈合的影响。方法 SPF级成年健康SD大鼠72只,麻醉状态下制作右侧胫中段骨折模型,模型制作完成后随机分为实验组和对照组,实验组术后每日灌服人工虎骨粉混悬液,对照组每日灌服蒸馏水。术后2周、4周、2月、3月分别进行影像学观察和组织学观察,术后2月、3月分别进行骨折侧生物力学测量、健侧生物力学测量,并计算骨折生物力学恢复率。结果影像学检查:术后4周,实验组骨痂形成较对照组更广泛、明显;术后2月,实验组皮质骨形成较对照组多。组织学观察:术后2周,实验组骨痂中软骨细胞较对照组明显多;术后4周,实验组骨小梁数量较多,骨痂改建较好;术后2月,实验组骨折处皮质层较厚度对照组大。生物力学测量:术后2月,骨折侧生物力学和骨折生物力学恢复率对照组分别为2.28±0.41N、40.94±10.55%,实验组分别为3.29±0.68N(P<0.05)、58.85±11.22%(P<0.05);术后3月,骨折侧生物力学和骨折生物力学恢复率对照组分别为2.67±0.52N、47.17±13.78%,实验组分别为4.14±0.47N(P<0.05)(P<0.05)、62.19±13.45%(P<0.05)。结论人工虎骨粉具有明显的促进骨折愈合作用,并可有效提高正常骨生物力学强度。
Objective To study the effect of artificial tiger bone on fracture healing in rats. Methods 72 healthy adult SPF level SD rats were middle fractured at the right tibia under anesthesia, and randomly divided into experimental and control groups. The experimental group was fed by artificial tiger bone suspension daily and distilled water for the control group. The histological imagings were observed after 2 weeks, 4 weeks, 2 months and 3 months respectively. The biomechanieal measurements at fracture side and the calculation of the recovery rate were performed after 2 month, 3 months each. Results Imaging： 4 weeks afteroperation,in the experimental group the callus formation is more extensive and obvious than the control group; 2 months postoperative, cortical bone formation in the experimental group more than control group. Histology： 2 weeks postoperative, in the experimental group the cartilage callus cells significantly more than the control group;while the experimental group show more trabecular bone volume and bone callus remodeling is better 4 weeks after operation; after 2 months, the experimental group, the fracture the thickness of cortical layers are better than the control group. Biomechanical measurements： 2 months afteroperation, the fracture mechanics and the recovery rate of the control group are 2. 28±0.41N ,40.94±10. 55% respectly and the experiment group are 3.29±0. 68N（P 〈0. 05） ,58.85±11.22% （P 〈0.05） ; 3 months after operation, the fracture mechanics and the recovery rate of the control group are 2. 67±0. 52N, 47.17±13.78% respectly and the experiment group are4. 14±0. 47N （ P 〈 0. 05 ） （ P 〈 0. 05 ） ,62.19±13, 45% （P 〈 0.05 ）. Conclusion The artificial tiger bone has a significant role in promoting fracture healing, and can effectively improve the strength of normal bone biomechanics.
出处
《中国骨质疏松杂志》
CAS
CSCD
2011年第7期605-608,616,共5页
Chinese Journal of Osteoporosis
基金
国家杰出青年科学基金(30625036)
国家自然科学基金(30801169)
国家自然科学基金(30971526)
国家自然科学基金(30973066)
北京市科技新星(A-2008-10)
首都医学发展科学基金(2007-2011)
国家科技部十一五科技支撑计划(2007BAI04B06)
关键词
人工虎骨粉
骨折
生物力学
大鼠
实验研究
Artificial tiger bone
Fracture
Biomechanics
Rat
Empirical study
