摘要
探讨抑癌基因P53突变在宫颈不典型增生形成中的作用和意义。方法：应用聚合酶链反应（PCR），单链构象多态性（SSCP）分析法检测了50例宫颈不典型增生、26例宫颈息肉、25例慢性宫颈炎症组织中P53外显子7－8的变异。结果：宫颈不典型增生、息肉、炎症组织中均未见P53外显子7－8缺失；宫颈不典型增生、息肉组织中P53外显子7－8突变阳性率分别为16％（8／50）、3．85％（1／26），二者差异显著（P＜0．001）；轻、中、重度不典型增生组织中突变无显著差异（P＞0．05）；慢性宫颈炎症组织中未见突变。结论：在宫颈不典型增生形成中P53外显子7－8的突变是一个重要且早期的因素。
To investigation the role and significane of P53 mutation in the genesis of cervical atypicalhyperplasia.Methods:P53(exon7 - 8 ) mutations were examined in 25 cervical atypical hyperplasia,26 cervical polrpand 25 cervical chronic inflamation by polyrnrase chain reaction (PCR) and single strand confonnaion polymrphism(SSCP). Resulte: The defects of P53 (exon7 - 8)were no foUn, but P53(exon - 8) mutations were detected in 4 of25(16% ) atypical hnperpalsia and in 1 of 26(3. 85% )polyp, and were not found in chronic infalmaion.About P53(exon7-8 ) mutations, there was a apparent difference (P<0.001). between atypical hyperplasia and polyp,but therewas no sighficant difference among light, moderate and geavy degree of atypical hyperplasia (P>0.05). Conclution:P53(exon7-8) mutations in the peis of cervical atypical hyperplasia is a important and early factor.
出处
《长治医学院学报》
1999年第4期248-249,共2页
Journal of Changzhi Medical College
基金
山西省自然科学基金
