摘要
使用DNAMAN软件分析β-苦瓜蛋白质氨基酸组成,亲水、疏水性,用生物信息学在线网站预测其基本特性,二、三级结构,卷曲螺旋区域,PEST序列,并用生物信息学软件分析其表面电位、可及性,建立Ramachandran图对模建结果进行结构质量分析与检测评价.结果发现:β-苦瓜蛋白质有249个氨基酸,等电点9.19,亲水区域和疏水区域交错分布;消光系数为280 nm,属于稳定蛋白质,平均亲水性是-0.138;二级结构中α-螺旋占42.57%,延伸链占20.48%,不规则卷曲占36.95%;分布着3段卷曲螺旋区域,存在5段非PEST序列,无稳定二聚体、三聚体卷曲螺旋;β-苦瓜蛋白质的三级结构、表面电位和可及性的空间分布得到了模建,Ramachandran图检测表明此模型结构符合立体化学规则.
The amino acid composition and hydrophilism and hydrophobicity of β-momorcharin were analyzed by DNAMAN software,and its basic features,secondary and tertiary structures,a coiled coil area and a PEST sequence were predicted by bioinformatics online websites,and the surface potential and accessibility were analysed by bioinformatics software,then the structural quality analysis and detection evaluation on the modeling result were carried out by establishing a Ramachandran graph.The result showed that the β-momorcharin comprised 249 amino acids and had isoelectric points of 9.19,and the hydrophilic area and the hydrophobic area were distributed in a staggered manner;the β-momorcharin belonged to stable protein as the extinction coefficient thereof was 280 nm,and the average hydrophilism was-0.138;the secondary structure comprised 42.57% of α-helix,20.48% of extended strand and 36.95% of irregular coil;there were three sections of coiled coil areas,five sections of non-PEST sequences,and no stable dipolymer or tripolymer coiled coil;the space distribution of tertiary structure,surface potential and accessibility of β-momorcharin were modeled,and the Ramachandran graph detection showed that the structure of the model met the rules of stereochemistry.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2011年第4期399-406,共8页
Journal of Zhejiang University:Agriculture and Life Sciences
