摘要
目的:构建烟草根特异性启动子植物表达载体并转入红景天发状根中。方法:以双元表达载体pCAM-BIA1301为基础,用烟草根特异性启动子TobRB7和葡萄糖基转移酶基因UGTR分别取代双元表达载体中的CaMV35S启动子和GUS基因,从而获得了烟草根特异性启动子驱动葡萄糖基转移酶基因的表达载体,命名为pCA-Tob7:UGTR。通过根癌农杆菌介导法转化红景天发状根。结果:成功地获得了转基因阳性发状根,即表明所构建的表达载体已成功整合到了红景天发状根基因组中。结论:首次获得了根特异性启动子植物表达载体并整合到了红景天发状根基因组中,为进一步研究特异性启动子对红景天苷合成的调控作用奠定了基础。
Objective：To construct root-specific promoter of plant expression vector and transformate into Rhodiola hairy root.Methods：The expression vector pCA-Tob7：UGTR,driven by tobacco root-specific promoter TobRB7,was constructed from pCAMBIA1301 by substituting the CaMV 35S promoter and GUS gene with TobRB7 and UGTR（a glycosyltransferase gene）,respectively.The pCA-Tob7：UGTR vector was introduced into Agrobacterium tumefaciens strain,and the hairy root of Rhodiola were then transformed.Results：Some Kanamycin resistant plants were positive,which indicated that the expression vector had integrated into Rhodiola hairy root genome.Conclusion：Root-specific promoter of plant expression vector is constructed and the hairy root of Rhodiola are transformed for future research.
出处
《中药材》
CAS
CSCD
北大核心
2011年第6期864-868,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金项目(30900112)
吉林农业大学科研启动基金(205-00369)
关键词
红景天发根
根特异性启动子
表达载体
转基因
Rhodiola hairy root
Root-specific promoter
Expression vector
Transgene
