摘要
以成年SD大鼠为实验材料,采用D-半乳糖连续腹腔注射制作亚急性衰老的大鼠模型,造模成功后连续灌胃蜂王浆,运用Morris水迷宫观察各组大鼠的空间学习记忆行为;荧光分光光度法检测各组大鼠脑组织中单胺类神经递质去甲肾上腺素(NE)、多巴胺(DA)的含量;检测各组大鼠脑组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量。结果显示:D-半乳糖衰老模型大鼠的空间学习记忆能力明显下降,与正常组大鼠相比,潜伏期由15.52s延长至21.62s,在探索实验中,穿过原站台的次数明显减少,由7.5次减少至4.25次(P<0.05),游泳轨迹类型发生明显变化;与正常组相比模型大鼠脑组织中NE、DA含量显著降低,SOD活性显著降低,MDA含量显著升高(P<0.05)。蜂王浆组大鼠空间学习记忆能力明显提高,与模型组相比,潜伏期由21.62s缩短至14.34s(P<0.05);其中脑组织NE、DA含量提高,与模型组相比,低剂量组有显著性差异(P<0.05),高剂量组有极显著性差异(P<0.01);SOD活性显著增高,MDA含量显著降低(P<0.05)。结果表明:蜂王浆可提高模型大鼠的空间学习记忆能力及脑组织中单胺类神经递质的含量、SOD活性,降低MDA含量。由此推测,蜂王浆可调节神经递质的合成及提高学习记忆能力,具有良好的延缓衰老、提高记忆的保健作用。
Adult SD rats were used as experimental subjects to establish a subacute aging model by intraperitoneally injecting D-galactose.After the aging model was successfully established,the aged rats were administered intragastrically with royal jelly.The learning and memory of the rats in each group were evaluated by Morris water maze.The contents of monoamine transmitters such as norepinephrine（NE） and dopamine（DA） in the brain tissue were determined in a fluorescence spectrophotometer.Meanwhile,cerebral SOD activity and DNA content in the rats were also determined.The results showed that the learning and memory of aged rats revealed a significant decrease when compared with those of normal rats.The average latency of the rats from the model group exhibited an obvious extension,which was prolonged from 15.52 s to 21.62 s.Meanwhile,the number of traversing original platform was dropped obviously,which was dropped from 7.5 to 4.25（P 0.05）.Moreover,the swimming track of the rats from the model group also exhibited a remarkable change.The contents of NE and DA,and the activity of SOD in the brain tissue of aged rats were significantly decreased;in contrast,the content of MDA was increased greatly（P 0.05）.On the other hand,the learning and memory of the rats from the royal jelly group were improved significantly when compared with that of aged rats from the model group.The average latency was shortened from 21.62 s to 14.34 s（P 0.05）,the contents of NE and DA in the brain and the activity of SOD were enhanced,but the content of MDA was dropped obviously.These results indicated that royal jelly can improve spatial learning and memory performance of aged rats and the contents of monoamine neurotransmitters as well as SOD activity,and reduce MDA content.Therefore,royal jelly can regulate the metabolism of monoamine transmitters to result in its anti-aging function and improve learning and memory capability.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第15期269-272,共4页
Food Science
