摘要
目的观察大鼠感染弓形虫后的学习记忆能力变化,并观测大鼠血清及脑组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、NO的变化。方法 48只雄性Wistar大鼠,分成弓形虫组与对照组,每组24只,弓形虫组腹腔注射2×107个/mL R H株弓形虫速殖子悬液2 mL,对照组腹腔注射灭菌生理盐水2 mL弓形虫组分别于感染后1、2、4、8周进行行为学测试,并检测血清及脑组织中SOD、MDA、NO含量。对照组大鼠分别于相同时间段检测上述指标。结果弓形虫与对照组大鼠时间、距离逃避潜伏期分别为(31.1±1.6)、(19.6±2.8)s和(1 066±3.2)、(772.0±6.9)cm,与对照组比较,弓形虫组大鼠潜伏期明显延长(P<0.05);弓形虫组大鼠血清、脑组织SOD分别为(91.2±8.3)、(78.9±3.2)U/mL,明显低于对照组(P<0.05);弓形虫组大鼠血清、脑组织MDA、NO含量分别为(22.1±1.3)、(7.8±2.0)nmol/mL和(21.5±3.1)、(29.9±1.8)μmol/mL,明显高于对照组(P<0.05)。结论大鼠感染弓形虫后学习记忆能力降低,可能与血清及脑内SOD活性降低、MDA、NO含量增高有关。
Objective To observe the change of the ability of learning and memory in the rats infected with Toxoplasma gondii（T.gondii） and to examine the contents of superoxide dismutase（SOD）,malodialdehyde（MDA）,and nitric oxide（NO） in peripheral blood and brain tissue of the rats.Methods Totally 48 male Wistar rats were randomly devided into a group with T.gondii infection and a control group without the infection.Suspension of T.gondii（2×107/ml,2ml） was injected intra-peritoneally into the rats in the experimental group.Then Morris water maze test was performed to observe the behavior changes of the rats at 1,2,4,8 week after the injecttion.The levels of SOD,MDA,and NO in the peripheral blood and brain tissue were tested.Results The average time and the average distance for the rats in test group was 31.1±1.6 s and 1 066±3.2 cm,and those of the control group were 19.6±2.8 s and 772.0±6.9 cm,with significant differences between the tow groups（P0.05）.The levels of SOD in peripheral blood and brain tissue were 91.2±8.3 U/mL and 78.9±3.2 U/mL for the rats of the test group and those of the control groups were 138.6±10.4 U/mL and 111.6±5.3 U/mL and those of the test group markedly decreased than the control group（P0.05）.The levels of MDA in peripheral blood and brain tissue of the test group were 22.1±1.3 nmol/mL and 7.8±2.0 nmol/mL,and those of the control group were 14.5±1.9 nmol/mL and 5.0±1.2 nmol/mL.The level of NO in peripheral blood and brain tissue of the test group were 21.5±3.1 μmol/mL and 29.9±1.8 μmol/mL,and those of the control group were 14.0±2.6 μmol/mL,19.1±2.2 μmol/mL.Compared with the control group,the concentration of MDA and NO in the test group increased markedly（P0.05）.Conclusion Infection with T.gondii can induce harmful effects on the ability of learning and memory in rats obviously.In addition,T.gondii can induce the decrease of SOD and the increase of MDA and NO,which may be one of the mechanism of T.gondii-induced behavior change in rats.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2011年第8期1010-1012,共3页
Chinese Journal of Public Health
关键词
弓形虫感染
学习记忆能力
MORRIS水迷宫
氧化应激
Toxoplasma gondii infection
learning and memory ability
Morris water maze
oxidative stress
