摘要
糖芯片是一种快速、高效、高通量获取糖-生物大分子相互作用信息的生物检测技术,对后基因组时代糖生物学的发展具有重大影响。本文主要论述了糖芯片固定化技术的最新进展,包括未经化学修饰的糖的化学固定,天然糖库及其固定,复杂寡糖的合成及其固定,糖基的密度差异固定化技术以及间隔基的引入技术。这些新的固定化技术保持了糖的化学结构,扩大了糖芯片的来源和应用范围,进一步提高了糖芯片的检测效率。此外,本文还介绍了虚拟筛选技术在这一领域的应用潜力以及糖芯片在医疗诊断等方面的应用,最后对糖芯片技术遇到的挑战和发展做了展望。
Carbohydrate microarrays, also referred to as glycan arrays, are new kinds of biological detection technology, providing a rapid, efficient, and high-throughput approach to examining carbohydrate-macromolecule interactions. This technology has had a significant impact on glyeobiology in the post-genomic era. This article focuses on recent advances in immobilization techniques which include covalently immobilizations of diverse unmodified glycans and natural glycan libraries, synthesis and immobilizations of oligosaccharides, strategies for varying carbohydrate density, and the insertion of spacer. These methods have advantages of maintaining the reducing sugar groups in their cyclic forms and expanding the complexity and the utility of glycan microarrays, which further enhance the detection efficiency of this technology. Moreover, the future potential of virtual screening in glycan array technology and practical applications of carbohydrate microarrays in clinical diagnostics are provided. Finally, limitations and the future trend of carbohydrate microarrays are also discussed.
出处
《化学进展》
SCIE
CAS
CSCD
北大核心
2011年第8期1719-1727,共9页
Progress in Chemistry
基金
国家自然科学基金项目(No.50403017)资助
关键词
糖芯片
糖生物学
固定化技术
虚拟筛选
carbohydrate microarray
glycobiology
immobilization techniques
virtual screening
