期刊文献+

外源蛋白在CHO细胞株中高效表达的策略 被引量:1

下载PDF
导出
摘要 目的:随着基因工程技术的不断发展,目前已有多种表达系统可用于生产具有医疗价值的人或动物来源的蛋白质。真核细胞中CHO细胞与其他表达系统相比具有许多优点,是目前重组糖基蛋白生产的首选体系。本文着重阐述外源蛋白在CHO细胞中高效表达的策略。
出处 《黑龙江医药》 CAS 2011年第4期561-563,共3页 Heilongjiang Medicine journal
  • 相关文献

参考文献12

  • 1Kim N S, Lee G M, Overexpression of bcl -2 inhibits sodium buty- rate - induced apoptosis in Chinese hamster ovary cells resulting in enhanced humanized antibody production [ J ]. Biotechnol Bioeng, 2001,71:184 - 193.
  • 2Pak S C O,Hunt S M N,Bridge M W et al. Super- CHO -A cell line capable of autocrine growth for fully deigned protein free con- ditions[ J ]. Cytotechnol, 1996,22 : 139 - 146.
  • 3Fussenegger M , Schlatfer S , Dawyler D , et al. Controlled pro- liferation by multigene metabolic engineering enhances the produc- tivity of Chinese hamster ovary cells. Nat Biotechnol , 1998 , 16 (5) : 468-472.
  • 4Timchenko N A , Wilde M , Nakakishi M , et al. CCAAT/en- haneer- binding protein alpha ( C/ EBP alpha ) inhibits cell proliferation through the p21 ( WAF - 1/CIP - 1/SDI - 1 ) pro- tein. Genes Dev . 1996 . 10(7):804-815.
  • 5Uetsuki T, Natio A, Nagata S, et al. Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor 1 alpha[ J ]. J Biol Chem, 1989,264 (10) : 5791 - 5798.
  • 6吴乃虎.基因工程原理(第二版)[M].科学出版社,2003.
  • 7Sadofsky M , Connelly S , Manley J L , et al. Identification of a sequence element,on the 3'side of AAUAAA which is necessary for simian virus 40 late mRNA 3' - end processing. Mol Cell Biol , 1985 , 5 (10) : 2713-2719.
  • 8Kaufman R J , Sharp P A , Latt S A. Evolution of chromosomal regions containing transfected and amplified dihydrofolate reduc- tase sequences. Mol Cell Biol, 1983 , 3 (4) :699-711.
  • 9Marilyn Kozak. At least six nucleotides preceding the AUG initia- tor codon enhance translation in mammalian cells[ J]. J Mol Biol, 1987,196:947 - 950.
  • 10Wemer R G. Appropriate mammalian expression systems [ J]. Drug Res, 1998, 48(8):870-880.

同被引文献11

引证文献1

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部