摘要
利用PCR技术从马铃薯陇薯3号基因组DNA中扩增出长度约为1.0 kb的DNA片段,经与T载体连接,测序表明,克隆到的DNA片段大小为969 bp,该序列与GenBank中已公布的patatin启动子序列同源性为97.94%;采用植物顺式调控元件数据库PLACE和P lantCare进行序列分析,结果表明,该片段含有启动子的保守序列TATA-box和CAAT-box,且在CAAT-box上游有8 bp的增强子。此外,还具有马铃薯块茎蛋白patatin基因启动子保守调控序列的蔗糖效应元件(SURE)4个及马铃薯储藏物特异结合调控patatin蛋白表达位点(B-box)2个,而这些特异序列可能是基因特异表达所必须的。
The patatin gene promoter region was amplified from Solanum tuberosumn genomic DNA by polymerase chain reaction（PCR）and cloned into the T-vector.The sequence analysis showed that the size of sequence is 969 bp,and its homology was 97.94% with published sequence of the patatin promoter in GenBank.Using PLACE and PlantCare sequence database analysis showed that the fragment containing the conserved promoter sequence,such as TATA-box,CAAT-box and enhancer element motif upstream of CAAT-box.In addition,the fragment containing four Sucrose Responsive Elements（SURE）of conserved among genes regulated by sucrose in the patatin gene promoter of potato and tow B-boxes of involved in potato tuber-specific and sucrose-inducible gene expression,these specific gene sequences may be necessary for specific expression.
出处
《生物技术通报》
CAS
CSCD
北大核心
2011年第7期69-72,共4页
Biotechnology Bulletin
基金
陕西省教育厅省级重点实验室重点科研计划项目(2010JS065)
延安市科技发展计划项目(2010kn-03)
延安大学专项科研基金项目
延安大学大学生科技创新训练项目(YD2008-106)
