摘要
目的观察大鼠肝纤维化形成过程中胰岛素样生长因子结合蛋白-2(IGFBP-2)的动态变化,探讨IGFBP-2与肝纤维化的关系。方法将36只大鼠随机分为2组:正常对照组6只(n=6),模型组30只(n=30)。除正常对照组外,给予大鼠40%CCl4皮下注射,每周2次,共计8周。正常对照组给予相同剂量的油剂皮下注射。模型组在开始注射CCl4后的第2、4、6、8周末分别处死大鼠6只。测定肝功能(ALT)、门静脉压,对肝组织进行常规HE与Masson三色染色,对血清中IGFBP-2采用酶联免疫法测定,并将其与肝纤维化程度进行相关分析。结果与正常对照组比较,2、4、6、8周模型组大鼠血清IGFBP-2水平增高(P<0.01)。模型组大鼠随着造模时间的延长,肝纤维化程度的加重,门静脉压逐渐增高,血清中IGFBP-2的水平逐渐增高,且IGFBP-2水平与肝纤维化程度呈正相关(r=0.874 2,P<0.001)。结论在肝纤维化形成过程中,血清中IGFBP-2的水平逐渐增高,提示其可能与肝纤维化有关。
Objective To investigate the dynamic changes of insulin-like growth factor binding protein-2（IGFBP-2） during the development of liver fibrosis and to explore the association between IGFBP-2 and liver fibrosis. Methods Thirty-six Wistar rats were randomly allocated into normal group（n=6） and model group（n=30）.Rats were given intraperitoneal injection of 40% CCl4 twice a week,totally for 8 weeks to induce the liver fibrosis in model group,while in control group the rats were subcutaneously injected with the same dosage of oil.Six rats in model group were killed at weeks 2,4,6 and 8,respectively.Portal vein pressure and ALT were examined in all rats.Histological changes of liver tissue were observed after hematoxylin and eosin（HE） staining and Masson staining.IGFBP-2 in serum was determined by enzyme-linked immunosorbent assay（ELASA）.The correlation between the IGFBP-2 and the degree of liver fibrosis was analyzed. Results The IGFBP-2 level was significantly higher in model group than in normal group（P0.01）.With the increase of modeling time,the liver fibrosis became worse,portal vein pressure and the level of IGFBP increased.The level of IGFBP was positively correlated with liver fibrosis degree（r=0.874 2,P0.001）. Conclusion The results suggest that the development of hepatic fibrosis may be related to the increase of IGFBP-2 in blood serum.
出处
《山西医科大学学报》
CAS
2011年第7期532-534,611,共4页
Journal of Shanxi Medical University
关键词
肝纤维化
肝功能
门静脉压
IGFBP-2
hepatic fibrosis
liver function
portal vein pressure
insulin-like growth factor binding protein-2
