摘要
将金纳米棒(AuNRs)和辣根过氧化物酶(HRP)以自组装的方式依次修饰到Au电极表面,构建了响应过氧化苯甲酰(BPO)生物传感器。采用循环伏安法和电流时间法研究了传感器的电化学性质和最佳工作条件;由于HRP/AuNRs复合膜的协同效应,电极性能得到显著改善。在最佳工作条件下(工作电压-0.02V,pH 7的磷酸盐缓冲体系),BPO浓度在5.0×10-6～1.0×10-4 mol/L范围内与电极的电流响应值呈良好的线性关系,线性回归方程为:i(μA)=12.6796C(mmol/L)+0.2406,R=0.9993。电极的检出限为8.5×10-7 mol/L。电极用于面粉中BPO的测定,平行测定6次,平均回收率为97.9%～100.1%,相对标准偏差(RSD)为0.5%～2.1%。本方法用于商品面粉中的BPO测定,取得满意结果。
A novel benzoyl peroxide（BPO） biosensor was prepared by self-assembling of gold nanorods（AuNRs） and horseradish peroxidase（HRP） on the surface of gold electrode by layer-by-layer.The electrocatalytic behavior and the optimum conditions of the biosensor were investigated by cyclic voltammetry and i-t curves in PBS.Taking the advantages of synergistic effect between AuNRs and HRP,the performance of the biosensor has been improved remarkably.Under the optimum conditions,-0.02 V and PBS pH 7,the current response of the biosensor was linearly proportional to BPO concentration ranged from 5.0×10-6 mol/L to 1.0×10-4 mol/L with a correlation coefficient of 0.9993 for benzoyl peroxide determination,and a detection limit of 8.5×10-7 mol/L.The linear regression equation was i（μA）=12.6796C（mmol/L）＋0.2406.The recovery of the BPO in flour and the RSD were 97.9%-100.1% and 0.5%-2.1% with 6 times determinations,respectively.The method was applied to the determination of BPO in flour in the market with satisfactory results.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2011年第8期1171-1175,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.21075035)
湖南大学化学生物传感与化学计量国家重点实验室基金(No.2008006)
湖南省自然科学基金(No.07JJ6027)
湖南省高校创新平台开放基金(No.10k024)资助项目
