摘要
采用对峙培养法从湖南浏阳地区烟田采摘的健康烟叶中筛选分离出86株菌株,其中B17菌株对烟草赤星病菌拮抗作用最强,抑制率为72.1%。通过生理生化实验和形态观察,初步鉴定B17菌株为短芽孢杆菌。在B17菌株发酵液的离体防效和温室栽培防效试验中,发酵液可以明显的减轻病害的发生,盆栽试验中,其防效为71.2%。
Eighty-six strains were isolated and selected from the healthy tobacco leaves collected from Liuyang area of Hunan Province by using confront culture method,in which the B17 showed the strongest antagonism to Alternaria alternata（Fries） Keissler with inhibition rate of 72.1%.Through physiological and biochemical experiments and morphologic observation,the B17 was identified as Bacillus brevis.The control efficacy tests of in vitro culture and green house culture for B17 zymotic fluid showed that the B17 zymotic fluid can obviously reduce the incidence of disease.In pot experiment,the control efficacy of the B17 zymotic fluid reached 71.2%.
出处
《湖南农业科学》
2011年第7期100-101,104,共3页
Hunan Agricultural Sciences
基金
湖南省烟草公司烟草赤星病的生物防治研究(SR0467)
关键词
赤星病
拮抗菌
发酵液
Alternaria alternata（Fries） Keissler
antagonistic bacterium
zymotic fluid
