摘要
研究豆类丝核菌次级代谢产物对鸡免疫功能的影响。试验一将200只14日龄健康未免疫雏公鸡随机分为8组,Ⅰ-Ⅳ组动物于14日龄以新城疫活疫苗滴鼻进行第1次免疫,2周后加强免疫1次,Ⅴ-Ⅷ组不免疫。Ⅰ-Ⅲ组、Ⅴ-Ⅶ组为试验组,分别注射高、中、低(1、2、4 mg/kg)3种剂量的豆类丝核菌次级代谢产物。Ⅳ组和Ⅷ组分别为疫苗对照组和空白对照组。于首免后连续6周各组随机抽取10只鸡进行ND-HI抗体效价检测;于首免后连续5周测外周血中白细胞数;于3、5、7周龄检测免疫器官(胸腺,脾脏,法氏囊)指数。试验二将20只30日龄健康已免疫雏公鸡随机分为4组用MTT法检测脾脏淋巴细胞的增殖活化。中剂量免疫试验组各试验指标与疫苗对照组相比均显著提高,中剂量未免疫试验组各试验指标与空白对照组相比均显著提高,高、低剂量试验组与疫苗对照组相比各试验指标无显著差异。
To study the Effects of the Rhizoctonia leguminicola secondary metabolite on the immune function of chicken.Text one：divided 200 14-days-old healthy chiken without immunity into 8 groups,groupⅠ-Ⅳ were Intranasal immuned with live ND vaccine at 14-days-old,strengthen the immune after 2 weeks,group Ⅴ-Ⅷ were not immuned.Group Ⅰ-Ⅲ、Ⅴ-Ⅶ were the tesr groups,injected high、middle、low dose（1、2、4 mg/kg）Rhizoctonia leguminicola secondary metabolite respectively.Group Ⅳ and group Ⅷ were the vaccine control group and blank control group.6 weeks after the first immune,extract 10 chiken from each group to test thier ND-HI antibodytiter;test thier white blood cell count in MPB for 5 weeks after the first immune;test their immune organ（spleen、thymus、bursa of Fabricius） index at 3-weeks-old、5-weeks-old、7-weeks-old.Test two：divided 20 30-days-old healthy immuned chiken into 4 groups,test their LYM proliferative activity by MTT method.All test index of middle dose immuned group is high obviously compare with the vaccine control group.All test index of middle dose nonimmune group is high obviously compare with the blank control group.High dose test group and low dose test group is no significant differences compare with the vaccine control group.
出处
《动物医学进展》
CSCD
北大核心
2011年第7期49-54,共6页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(30270979)
