摘要
研究了铝胁迫下耐铝性不同的两个小麦品种根细胞液泡膜ＡＴＰ 酶、焦磷酸酶活性和膜脂的变化。与对照相比，经２０ 和１００μｍｏｌ／Ｌ的ＡｌＣｌ３ 处理后，耐铝品种Ａｌｔａｓ ６６ 的液泡膜Ｈ＋ＡＴＰ 酶和Ｃａ２＋ＡＴＰ 酶活性迅速下降； 铝敏感品种Ｓｃｏｕｔ ６６ 液泡膜Ｈ＋ＡＴＰ酶和Ｃａ２＋ＡＴＰ 酶活性则在２０ μｍｏｌ／Ｌ 时增加，１００ μｍｏｌ／Ｌ时下降。焦磷酸酶活性在Ａｌｔａｓ６６ 中下降，在Ｓｃｏｕｔ ６６ 中增加。与对照相比，在ＡｌＣｌ３ ２０ μｍｏｌ／Ｌ处理时，液泡膜磷脂含量增加，Ａｌｔａｓ ６６ 中的增加比Ｓｃｏｕｔ ６６ 更为明显；１００ μｍｏｌ／Ｌ 时，Ｓｃｏｕｔ ６６ 液泡膜磷脂含量迅速下降，而Ａｌｔａｓ ６６ 下降不显著。两品种小麦在铝处理后根液泡膜糖脂结合半乳糖含量均高于对照，而Ａｌｔａｓ ６６ 中的含量又高于Ｓｃｏｕｔ６６ 。铝处理后，两品种小麦根液泡膜的棕榈酸和油酸含量增加，亚麻酸含量下降， 不饱和指数也随之下降，其中Ｓｃｏｕｔ６６ 下降更为明显。表明Ａｌｔａｓ ６６ 根细胞液泡膜比Ｓｃｏｕｔ６６ 对铝胁迫有更强的适应能力。
This paper deals with the changes in ATPase and PPase activities, lipid compositon of tonoplast vesicles from two wheat(Triticum aestivum) cultivars, Altas 66(Al tolerant) and Scout 66(Al sensitive) under Al stress. In comparison with the controls, the activities of H + ATPase and Ca 2+ ATPase of tonoplast vesicles in Altas 66 were decreased greatly by AlCl 3 20 and 100 μmol/L treatment, those of Scout 66 were increased at AlCl 3 20 μmol/L,but it decreased at AlCl 3 100 μmol/L(Figs.1, 2).The H + PPase activity of Altas 66 was decreased by Al treatment, while that of Scout 66 increased(Fig.3). The phospholipid contents of tonoplast vesicles in Altas 66 and Scout 66 were increased by AlCl 3 20 μmol/L treatment,but it was decreased by AlCl 3 100 μmol/L(Fig.4).The contents of galactose in lipids of two cultivars after Al treatments was higher than those of the controls, and it was higher in Altas 66 than in Scout 66(Fig.5). After Al treatment, the palmitic acid and stearic acid contents increased, the linolenic acid content and the unsaturation index of fatty acids decreased in both cultivars, but those in Scout 66 more distinctly(Table 3). To sum up, the tonoplast of roots of Altas 66 showed a higher adaptability to Al stress than that of Scout 66.
基金
国家自然科学青年基金
关键词
铝胁迫
液泡膜
ATP
焦磷酸酶
膜脂
小麦
Al stress, tonoplast, H^+ ATPase, Ca^(2+) ATPase, H^+ PPase, lipid composition
