摘要
目的探讨盐酸戊乙奎醚(PHC)预处理对大鼠急性肺损伤支气管肺泡灌洗液TNF-α和IL-6水平的影响。方法 48例SD大鼠,随机分4组:C组:尾静脉注射生理盐水1ml/kg;M组:尾静脉注射大肠杆菌脂多糖(LPS)5mg/kg;P1组:尾静脉注射大肠杆菌LPS 5mg/kg+PHC 0.25mg/kg;P2组:尾静脉注射大肠杆菌LPS5mg/kg+PHC 0.75mg/kg。每组12只。观察与C组和M组比较,支气管肺泡灌洗液TNF-α和IL-6结果。结果 TNF-α:与C组比较,3组升高(P<0.01);与M组比较,P1和P2组降低(P<0.01);与P1组比较,P2组降低(P<0.05)。IL-6:与C组比较,3组升高(P<0.01);与M组比较,P1和P2组降低(P<0.01);与P1组比较,P2组降低(P<0.05)。结论 PHC预处理能有效减低脓毒症大鼠肺组织血浆TNF-α和IL-6水平,并且在一定剂量范围内呈剂量效应关系。
Objective To study the influence and relationship of TNF-α and IL- 6 in BALF of Penehyelidine Hydrochloride（ PHC ） on rats with acute lung injury （ALI）. Methods Totally 48 SD rats are divided into four groups randomly and there are 12 rats in each group. C group： tail vein injection of saline 1 ml/kg ; M group ： tail vein injection of E. coli Lipopolysacharide （LPS） 5 mg/kg ; P1 ： tail vein injection of E. coli LPS 5mg/kg and PHC 0.25mg/kg; P2： tail vein injection of E. coli LPS 5mg/ kg and PHC 0. 75mg/kg. Results TNF - alpha： Compared with C group, the rest 3 group increased significantly （ P 〈 0. 01 ）. TNF - alpha in both P1 and P2 group were higher than that in M group （P 〈0. 01 ）. Compared with P1 group, TNF -alpha in P2 group reduced significantly （P 〈0. 01 ）. IL - 6： Compared with C group, BALF IL -6 among M, P1 and P2 group increased significantly （P 〈 0. 01 ）. IL - 6 in both P1 and P2 group were higher than that in M group （P 〈 0. 01 ）. Compared with P1 group, IL - 6 in P2 group reduced significantly （P 〈 0. 01 ）. Conclusions The intravenous injection of PHC can effectively reduce TNF - α and IL - 6 of BALF in septic rats with ALI and showed a definite doseeffect relationship.
出处
《医药论坛杂志》
2011年第11期77-80,共4页
Journal of Medical Forum
关键词
盐酸戊乙奎醚
急性肺损伤
炎性反应
Penehyclidine hydrochloride （PHC）
Acute lung injury （ALI）
Inflammatory response
